一测多评法测定藏族药肉果草中4种成分的含量

目的:建立藏族药肉果草中4种成分的一测多评定量测定方法,考察不同类型化合物之间采用相对校正因子(relative correction factors,RCF)进行含量测定的准确性和可行性。方法:以藏族药肉果草为研究对象,建立内参物毛蕊花糖苷与其他3个指标成分间的RCF因子,并利用RCF计算含量,实现一测多评。同时采用外标法测定这4个成分的含量,比较二者间的差异。结果:在一定的线性范围内,毛蕊花糖苷与梓醇、连翘苷、连翘脂素间的RCF分别为7.044 9,2.135 0,0.964 7;在不同试验条件下重复性良好(RSD分别为2.7%,1.2%,1.2%);且不同来源的肉果草中4个成分含量的一测多评法计算值与外标法测定结果间无明显差异。结论:肉果草中4个成分的一测多评法准确可行,适用于肉果草中成分的含量测定,为藏族药的多指标质量评价提供了新的参考。

Simultaneous Determination of 4 Constituents in Tibetan Herb-medicine Lancea tibetica by HPLC-QAMS

Objective: To establish a quantitative assay of multi-components by single marker (QAMS) for determination of four bioactive components in Lancea tibetica, and examine the accuracy and feasibility of the relative correction factors (RCFs) between different types of compounds. Method: L. tibetica was chosen as the research object, and RCFs were determined between acteoside and catalpol, phillyrin and phillygenol. The contents of the four constituents were also calculated according to the RCFs to carry out QAMS. Difference of QAMS and external standard method was investigated to validate the accuracy and feasibility of the QAMS method. Result: RCFs between acteoside and catalpol, phillyrin and phillygenol were 7.0449, 2.1350 and 0.9647 respectively within a certain linear range;repeatability was good in different experimental conditions (RSDs were 0.3%, 1.2% and 1.2% respectively);there was no significant differences between the quantitative results of QAMS method and external standard method for 4 constituents. Conclusion: It is accurate and feasible to evaluate 4 constituents of L. tibetica by QAMS. Therefore, this method is suitable to determine different types of components, and can provide a new reference for the quality assessment of Tibetan herb-medicine.