| 苦参碱对C57BL/6J小鼠Lewis肺癌VEGF表达的影响 |
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| 引用本文： | 郭斌,申洪波,彭宏伟,李智.苦参碱对C57BL/6J小鼠Lewis肺癌VEGF表达的影响[J].中国现代应用药学,2011,28(1):14-17. |
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| 作者姓名： | 郭斌 申洪波 彭宏伟 李智 |
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| 作者单位： | 1.辽宁医学院附属第一医院，辽宁 锦州 121000；;辽宁医学院附属第二医院，辽宁 锦州 121000;辽宁医学院，辽宁 锦州 121000;中国医科大学天然药物研究室，沈阳 110001 |
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| 基金项目： | 辽宁省科技厅第二批药物源头创新研究课题(2006226031-217) |
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| 摘 要： | 目的 研究苦参碱对小鼠Lewis肺癌的作用,并探讨其作用机制.方法 建立小鼠Lewis肺癌模型,将实验小鼠分为生理盐水组、不同剂量苦参碱组、环磷酰胺组,计算原发瘤抑制率及微血管密度;采用RT-PCR和Western blot法检测原发瘤组织VEGF mRNA和蛋白表达.结果 应用苦参碱各剂量组,小鼠原发瘤抑制率、微血管...
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| 关 键 词： | 苦参碱 Lewis肺癌 血管内皮生长因子 |
| 收稿时间： | 2010/5/20 0:00:00 |
Effect of Matrine on VEGF mRNA and Protein Expression Levels of Lewis Lung Carcinoma in C57BL/6J Mouse |
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| GUO Bin,SHEN Hongbo,PENG Hongwei and LI Zhi.Effect of Matrine on VEGF mRNA and Protein Expression Levels of Lewis Lung Carcinoma in C57BL/6J Mouse[J].The Chinese Journal of Modern Applied Pharmacy,2011,28(1):14-17. |
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| Authors: | GUO Bin SHEN Hongbo PENG Hongwei and LI Zhi |
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| Institution: | 1.The First Hospital Affiliated to Liaoning Medical College, Jinzhou 121000, China;;The Second Hospital Affiliated to Liaoning Medical College, Jinzhou 121000, China;;Liaoning Medical College, Jinzhou 121000, China;;Department of Ethnopharmacology, China Medical University, Shenyang 110001, China |
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| Abstract: | OBJECTIVE To investigate the effect of matrine on Lewis lung carcinoma in C57BL/6J mouse and to explore its molecurlar mechanism. METHODS The model of mouse with Lewis lung carcinoma was made, the experimental mice were randomly divided into normal saline group, matrine groups at varied concentrations and cytoxan group. Tumor inhibitory rate and microvessel density (MVD) were measured; the mRNA and protein expression of VEGF were determined with RT-PCR and Western blot. RESULTS There were significant differences from inhibitory rates of primary tumor and MVD between matrine groups and normal saline group(P<0.05 or P<0.01); VEGF mRNA and protein expression levels in matrine groups were significantly lower than in normal saline group. CONCLUSION Matrine could inhibit the growth of implanted Lewis lung carcinoma in C57BL/6J mouse at different levels and reduce MVD of Lewis lung carcinoma obviously, which was probably because that matrine reduced the expressions of VEGF mRNA and protein of Lewis lung carcinoma. |
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| Keywords: | matrine Lewis lung carcinoma vascular endothelial gronth factor |
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