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兔视网膜色素上皮细胞移植及长期形态学的研究
引用本文:叶俊杰 张承芬. 兔视网膜色素上皮细胞移植及长期形态学的研究[J]. 中华眼科杂志, 1995, 31(2): 109-113
作者姓名:叶俊杰 张承芬
作者单位:北京协和医院眼科
摘    要:视网膜色素上皮(RPE)功能失调或发生病理改变,可导致许多视网膜疾病,包括年龄相关性黄斑变性,青年性遗传性黄斑变性及视网膜色素变性等,严重危害人类视力。能否通过移植正常、健康的RPE细胞治疗这类视网膜疾病,是当前眼科界关注的重要研究课题。我们采用了改良式玻璃体密闭式移植术,术中增加了玻璃体切割,应用带有色素标记的兔RPE细胞移植于无色素性兔的视网膜下间隙。结果显示,移植长达1年后的RPE细胞不但存活,结构形态正常,并且与邻近的RPE细胞(包括移植的与自体的)建立了闭锁小带。移植的RPE细胞与受体的感光细胞紧密结合,受体眼的神经外节盘正常脱落,被移植的RPE细胞吞噬,且未发生排异反应。上述结果显示,本实验兔RPE细胞移植的成功,为临床治疗某些视网膜疾病开辟了新的治疗途径,提供了可靠的实验室根据和应用前景。

关 键 词:眼上皮 色素 受体 上皮细胞移植 形态学 视网膜

Long-term follow-up of allotransplantation of retinal pigment epithelial cells in rabbit with special refereuce to morphological studies
Ye Junjie,Zhang Chengfen,Ryan SJ. Long-term follow-up of allotransplantation of retinal pigment epithelial cells in rabbit with special refereuce to morphological studies[J]. Chinese Journal of Ophthalmology, 1995, 31(2): 109-113
Authors:Ye Junjie  Zhang Chengfen  Ryan SJ
Abstract:Dysfunction as well as pathological changes of retinal pigment epithelium(RPE)has been implicated as the causes of some retinal diseases, including age-related macular degeneration,hereditary macular degeneration and retinitis pigmentosa,etc.Transplantation of normal healthy RPE cells with an attempt to treat the retinal diseases is one of the current interests in ophthalmic research.We have developed a medified pars plana technique including vitrectomy for subretinal transplanta-tion of homologous,cultured, natural pigment labelled RPE cells in the non-pigmented rabbit.Our re-sults,with follow-up from one week to one year,revealed that the transplanted RPE cells not only sur-vived and attached to Brucr's membrane of the host eyes,but also had a distinct basal and apical mor- phology as seen in close contact with host phOtoreceptors,and formed junctional complexes with neigh-boring RPE cells.Close contact between the microvilli of the grafted RPE cells and the photoreceptor outer segments of the host was also observed.The normal periedical sheddings of the host outer seg-ments were phagocytosed by the transplanted RPE cells,as demonstrated in our results.Furthermore,there was practlcally no,if any,sign of rejection found.All the results have demonstrated that the transplantation of RPE cells in rabbits was successful and these observations provide a reliable experi-mental evidence for the clinical research of allotransplantation of RPE cells.
Keywords:Retinal pigment epithelial cells Rabbit Receptor  
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