人类晶体上皮细胞培养及其生长抑制的实验研究

对正常人晶体及白内障晶体上皮细胞进行体外培养，并观察体外培养的晶体上皮细胞的生物学特性及组织学变化。最后应用５－氟脲嘧啶、高三尖杉酯碱及骆驼蓬碱对组织培养的晶体上皮细胞进行药物抑制实验。结果显示，人类晶体上皮细胞在体外生存能力有限，体外的生长能力与供体年龄呈负相关。高三尖杉酯碱及骆驼蓬碱可有效地抑制晶体上皮细胞的生长繁殖。根据对培养的晶体上皮细胞生长特性的观察，提出使用边缘光滑的撕囊术可能会降低白内障术后后囊混浊的发生率。

Human lens epithelial cell culture and its proliferative inhibition in vitro

The cuItures of human lens epithelial cells taken from both normal and cataractous lenses were established in vitro.The biological characteristics and the histological changes of the cells were in-vestigated.5-Fluorouracil(5-Fu),Homoharring tonine(HH)and Harmeline(H)were used for the in-hibition of the proliferative cells in the experiment.The results revealed that the in vitro survival capaci-ty of the human lens epithelial cells is limited and the proliferative capacity of the cells is negatively cor-related to the donor age.HH and H can effectively inhibit the lens epithelial cell proliferation.Based on the results of this report,performing small and smcothly edged anterior capsulorhexis in extracapsular cataract extraction may possibly decrease the incidence of the post-operative opacification of the posterior capsule.