人巨细胞病毒感染对腮腺导管上皮细胞角蛋白K8和K18表达的影响

目的 研究人巨细胞病毒(HCMV)感染对腮腺导管上皮细胞角蛋白K8和K18表达的影响.方法 用免疫组化方法研究腮腺巨细胞包涵体病(PCID)石蜡包埋组织中巨细胞病毒立即早期抗原和角蛋白K8、K18的表达.结果 PCID腮腺组织导管上皮出现包涵体巨细胞;包涵体巨细胞HCMV抗原DDG9/CCH2阳性;包涵体巨细胞角蛋白K8表达呈阴性,而K18表达呈强阳性.结论 HCMV感染腮腺导管上皮细胞诱发角蛋白K8降解,K18表达上调是一种反应性改变;单层上皮角蛋白网具有维持上皮细胞机械力学完整性的功能.

Effect of HCMV on expressions of K8 and K18 in duct epithelial cells of salivary gland

Objective To study the effect of human cytomegalovirus (HCMV) on expressions of K8 and K18 in duct epithelial cells of salivary gland. Methods The expressions of immediate early antigen of HCMV, K8 and K18 were detected by immunohistochemistry staining in tissues embedded in paraffin of parotid cytomegalic inclusion disease(PCID). Results Cytomegly bearing inclusion appeared in duct epithelium of PCID. DDG9/CCH2 antigen of HCMV was expressed in cytomegly bearing inclusion. K8 was negative in these cytomegly while K18 was intensively positive. Conclusion It is suggested that breaking down of K8 be induced in parotid duct epithelial cells infected by HCMV and that up-regulation of K18 may be a reactive change. Keratin network in simple epithelium functions to impart mechanical integrity to cells.