| 转染pcDNA3-hCEA的人树突状细胞对MGC803细胞的作用 |
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| 引用本文： | 马俊芬,杨洪艳,赵明耀,黄幼田,郑智敏,董子明.转染pcDNA3-hCEA的人树突状细胞对MGC803细胞的作用[J].郑州大学学报(医学版),2005,40(6):1037-1038. |
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| 作者姓名： | 马俊芬 杨洪艳 赵明耀 黄幼田 郑智敏 董子明 |
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| 作者单位： | 郑州大学基础医学院病理生理学教研室,郑州,450052 |
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| 基金项目： | 河南省科技攻关基金资助项目 0324410034;河南省科技创新人才工程项目 20008402 |
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| 摘 要： | 目的：观察转染含人癌胚抗原（CEA）真核表达质粒pcDNA3-hCEA的人树突状细胞（DC）对胃癌细胞MGC803的抑制作用.方法：采用细胞因子rhIL-4和rhGM-CSF诱导培养法制备人外周血DC并用Lipofectamine向人DC转染pcDNA3-hCEA.RT-PCR检测转染细胞hCEA的表达,观察DC-hCEA细胞诱导的致敏的T淋巴细胞（CTL）对胃癌细胞MGC803的杀伤效应.结果：DC-hCEA诱导的CTL对胃癌细胞MGC803的杀伤效应强于DC（P＜0.05）.结论：编码CEA基因的真核表达质粒转染的人DC能够诱导较强的特异性抗肿瘤免疫效应.
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| 关 键 词： | 树突状细胞 癌胚抗原 胃肿瘤 MGC803细胞 |
| 收稿时间： | 2005-03-10 |
| 修稿时间： | 2005年3月10日 |
Antitumor effect of human dendritic cells transfected with pcDNA3-hCEA on MGC803 |
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| MA Junfen,YANG Hongyan,ZHAO Mingyao,HUANG Youtian,ZHENG Zhimin,DONG Ziming.Antitumor effect of human dendritic cells transfected with pcDNA3-hCEA on MGC803[J].Journal of Zhengzhou University: Med Sci,2005,40(6):1037-1038. |
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| Authors: | MA Junfen YANG Hongyan ZHAO Mingyao HUANG Youtian ZHENG Zhimin DONG Ziming |
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| Institution: | Department of Pathophysiology, College of Basic Medical Sciences, Zhengzhou University,Zhengzhou 450052 |
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| Abstract: | Aim: To observe the dendritic cell (DC) transfected with pcDNA3-hCEA and its immunity effect on MGC803. Methods: DC was generated from human fresh peripheral blood induced by rhGM-CSF and rhIL-4. DC vaccine was prepared by transfection with pcDNA3-hCEA using lipofection. The immunity effect of the DC on MGC803 was observed.Results: Compared with DC that was not transfectd,the antitumor immune response induced by DC-hCEA was more powerful(P<0.05). Conclusion: The results demonstrate that specific antitumor immune responses could be induced efficiently by vaccination of DC transfected with pcDNA3-hCEA. |
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| Keywords: | dendritic cell carcinoembryonic antigen MGC803 cell |
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