6种多糖对小鼠免疫细胞活性作用的比较研究

目的探讨香菇多糖等6种多糖(LPS)对小鼠免疫细胞活性的作用,并比较其免疫调节作用的途径和强度。方法6种多糖体外作用于小鼠免疫细胞,MTT法测脾淋巴细胞增殖活性,ELISA法测LPS诱导的脾淋巴细胞分泌IgG水平,吞噬中性红法测腹腔巨噬细胞吞噬功能,乳酸脱氢酶测定法测脾NK细胞活性。结果协同ConA促进脾淋巴细胞增殖作用强弱依次是香菇多糖、灵芝多糖、黄芪多糖、人参多糖、海带多糖和地衣多糖(P<0.05);协同LPS促进脾淋巴细胞增殖作用依次是灵芝多糖、香菇多糖、地衣多糖、黄芪多糖和人参多糖(P<0.05);促进脾淋巴细胞分泌IgG的依次是黄芪多糖、灵芝多糖、人参多糖、地衣多糖和>香菇多糖(P<0.05);增强腹腔巨噬细胞吞噬活性的依次是黄芪多糖、地衣多糖、香菇多糖、海带多糖、灵芝多糖和人参多糖(P<0.05);增强脾NK细胞活性的依次是黄芪多糖、地衣多糖、海带多糖、香菇多糖和灵芝多糖(P<0.05)。结论6种多糖作用的机制和效果各不相同,香菇多糖、灵芝多糖、地衣多糖和黄芪多糖分别在增强细胞免疫、体液免疫和非特异免疫的某一功能上效果显著,可以适宜的剂量比例组合成复合多糖。

Effects of six polysaccharides extracted from plants on the immunological cells of mice

Objective To compare the immunoenhancement of six polysaccharides on the murine immunological cells in vitro.Methods The immunoenhancement of the polysaccharide on the murine splenocytes were tested by MTT assay.The effects of polysaccharides on IgG secretion of spleen lymphocytes were detected by ELISA.The activation of peritoneal macrophages were tested by the method of neutral red phagocytosis assay.The phagocytic activity of spleen NK cells were detected by the method of LDH.Results Lentinan and Ganoderan could promote spleen lymphocyte proliferation with ConA significantly.Astragalan,Ganoderan and Lentinan had stronger function in promoting the IgG secretion and proliferation of spleen lymphocyte with LPS.Lichenan and Astragalan could significantly enhance activation of peritoneal macrophages and splenic NK.Conclusion The immuno-regulation mechanism and effects of six polysaccharides were indivadually variable.The polysaccharide aiming at different effect targets could be combined into the most immuno-potentiating agent by the most appropriate doses ratio.