Homologous linear plasmids in mitochondria of three species of wheat bunt fungi,Tilletia caries,T. laevis and T. controversa |
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| Authors: | W K Kim E Whitmore G R Klassen |
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| Institution: | (1) Agriculture Canada Research Station, 195 Dafoe Road, R3T 2M9 Winnipeg, Manitoba, Canada;(2) Department of Microbiology, University of Manitoba, R3T 2N2 Winnipeg, Manitoba, Canada |
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| Abstract: | Summary All isolates of Tilletia spp. investigated (five isolates of T. caries, including one from Japan, two isolates of T. laevis, and five isolates of T. controversa) contained a linear DNA plasmid ranging in size from 7.2 to 7.6 kb. All plasmids were highly homologous to each other as shown by DNA-DNA hybridization and comparison of restriction enzyme sites. Variability in the size of the plasmid was found to be due to differences within a central region of the plasmid. No homology between the plasmid and mitochondrial or nuclear DNA was found, but the mitochondrial origin of the plasmid was confirmed. |
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| Keywords: | Linear plasmids Mitochondria Wheat Bunt fungi Tilletia |
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