| 检测肿瘤McAb新技术DELFIA的建立 |
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| 引用本文： | 陈智周,范振符.检测肿瘤McAb新技术DELFIA的建立[J].中国医学科学院学报,1991,13(1):60-62. |
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| 作者姓名： | 陈智周 范振符 |
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| 作者单位： | 肿瘤研究所，肿瘤研究所，肿瘤研究所，Pharmacia CanAg，Gothenburg，Sweden，Pharmacia CanAg，Gothenburg，Sweden 北京，北京，北京 |
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| 摘 要： | DELFIA(dissociation enhancement lanthanide fluoroimmunoassay)是近年来发展的最新一代免疫分析技术,其全名为解离放大镧系荧光免疫分析法或Tr-FIA(time-resolved fluoroimmuno-assay),即时间分辨荧光免疫分析法,其与传统放射免疫法比较具有更高的灵敏度和精确度.作者从8611卵巢癌细胞株获得McAb F_(11)、C_4、F_(10)、H_1-F_8及H_1-F_7,并分别成功地建立了前4种McAb的DELFIA,本文报告F_(11)及G_4 DELFIA的建立.
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| 关 键 词： | 肿瘤 检测 诊断 McAb |
The development of DELFIA with novel tumor monoclonal antibodies E-11 and G-4] |
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| Z Chen.The development of DELFIA with novel tumor monoclonal antibodies E-11 and G-4][J].Acta Academiae Medicinae Sinicae,1991,13(1):60-62. |
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| Authors: | Z Chen |
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| Institution: | Institute of Oncology, Beijing. |
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| Abstract: | Monoclonal antibodies E-11 and G-4 were purified by Sepharose 4B column chromatography and labelled with europium as tracer. The solid-phase antibodies were prepared by attaching pure E-11 and G-4 to polysterene strips. The supernatant of 8611 cell culture or perchloric acid extracts from ovarian cancer tissues provided standard antigens. The solid-phase sandwich-type DELFIAs for monoclonal antibodies E-11 and G-4 were thus established, and the resulting standard curves were extremely steep. The ratio of CPS in high-concentration tubes to CPS in the zero tube exceeded 300:1, indicating the very high sensitivity and accuracy of this method. |
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