Dracorhodin perchlorate induces apoptosis in HL-60 cells |
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| Authors: | Xia M-Y Wang M-W Cui Z Tashiro S-I Onodera S Minami M Ikejima T |
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| Affiliation: | a Department of Pharmacology, Shenyang Pharmaceutical University, Shenyang, Chinab Department of Pharmacognosy, School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang, Chinac Department of Clinical and Biomedical Sciences, Showa Pharmaceutical University, Tokyo, Japand Department of Immunology, Yokohama City University School of Medicine, Yokohama, Japane China-Japan Research Institute of Medical and Pharmaceutical Sciences, Shenyang Pharmaceutical University, Shenyang, China |
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| Abstract: | Dracorhodin perchlorate, an anthocyanin red pigment, induces human premyelocytic leukemia HL-60 cell death through apoptotic pathway. Caspase -1, -3, -8, -9, and -10 inhibitors partially reversed the cell death induced by dracorhodin perchlorate. Caspase-3 and -8 were activated followed to the degradation of caspase-3 substrates, inhibitor of caspase-activated DNase (ICAD) and poly-(ADP-ribose) polymerase (PARP). Dracorhodin perchlorate up-regulated the expression ratio of mitochondrial proteins, Bax/Bcl-XL. The cell death was accompanied with phosphorylation of ERK, JNK and p38 MAPK and partially reduced by MEK inhibitor (PD98059), JNK MAPK inhibitor (SP600125) and p38 MAPK inhibitor (SB 203580). Taken together, dracorhodin perchlorate-induced apoptosis in HL-60 cells via up-regulation of Bax, activation of caspases and ERK/p38/JNK MAPKs. |
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| Keywords: | Dracorhodin perchlorate Human premyelocytic leukemia HL-60 cell Apoptosis |
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