蛇毒神经生长因子对大鼠视网膜缺血再灌注损伤后微管相关蛋白1B的影响

目的 探讨玻璃体腔内注射蛇毒神经生长因子是否对实验性大鼠视网膜缺血再灌注损伤的视网膜中的微管相关蛋白1B有影响而起到神经保护作用.方法 采用升高大鼠眼内压的方法,制作实验性视网膜缺血再灌注损伤模型.实验组和对照组分别注入蛇毒神经生长因子和平衡盐溶液,通过免疫组织化学法检测再灌注后不同时间段各组大鼠视网膜组织中微管相关蛋白1B的表达.结果 微管相关蛋白1B的表达在实验组于再灌注后6 h加强,48h达高峰.而对照组在再灌注后24h增强,96h才达高峰,而且高峰浓度实验组较对照组强(P<0.01).结论 通过向大鼠玻璃体腔内注射蛇毒神经生长因子可以提高视网膜微管相关蛋白1B的表达而对实验性视网膜缺血再灌注损伤有神经保护作用.

The effects of viper venom nerve growth factor on the expression of MAP1B on experimental retinal ischemia/reperfusion injury

Objective To evaluate" the effects of viper venom nerve growth factor (vNGF) on the expression of Mierotubule associated proteins 1B (MAP 1B) after vNGF was injected into the vitreous cavity on experimental retinal ischemia/reperfusion injury (RIR).Method The Spregue-Dawley (SD) rat model of RIR was made by increasing the intraoeular pressure.The rats were divided into normal,experimental and control groups randomly.At the beginning of reperfusion,20μ1 (100BU) of vNGF was injected into the experimental group and 20μ1 of Balanced Salt Solution (BSS) was injected into the vitreous cavity in Control group.The expression of MAPIB in retina at different time after reperfusion were observed.The expression of MAPIB was studied by immunohistochemistry.Result The expression of MAPIB was found in normal group.In experimental group the expression of MAP1B enhanced gradually at 6h after reperfusion,reached the peak at 48h.In Control group,the expression of MAPIB enhanced gradually at 24h after reperfusion,reached the peak at 96h, and at the same time the light density of stain was more weak than that at 48h in experimental group (P<0.01).The expression of MAP1B of experimental and control group decreased at 120h,and then the expression of MAPIB at 168h was similar as normal group.Conclusion Injection ofvNGF into the vitreous cavity has the protection effect on experimental retinal ischemia/repeffusion injury through enhancing the expression of MAP1B to promote the regeneration of retinal nerve fiber.