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| Neuropilin-1在小鼠黑色素瘤细胞增殖中的作用 | |
| 引用本文： | 裴向克,蔡明,周鸿敏,刘畅,徐利军,黄建敏,陈忠华,石炳毅. Neuropilin-1在小鼠黑色素瘤细胞增殖中的作用[J]. 现代肿瘤医学, 2010, 18(2) |
| 作者姓名： | 裴向克 蔡明 周鸿敏 刘畅 徐利军 黄建敏 陈忠华 石炳毅 |
| 作者单位： | 1. 解放军第281医院泌尿外科,河北,秦皇岛,066100;解放军第309医院全军器官移植中心,北京,100091 2. 解放军第309医院全军器官移植中心,北京,100091 3. 华中科技大学同济医学院附属同济医院器官移植研究所,湖北,武汉,430030 |
| 基金项目： | 国家自然科学基金资助项目，全军医学"十一五"计划科技攻关课题资助项目 |
| 摘 要： | 目的:探讨Neuropilin-1(Nrp-1,神经菌毛素)在小鼠黑色素瘤细胞体外增殖中的作用.方法:用活细胞荧光染料羧基荧光素乙酰乙酸琥珀酰亚胺酯(carboxyfluorescein diacetate succinimidyl ester,CFDA-SE)标记小鼠B16-F10黑色素瘤细胞后,将肿瘤细胞分别用Nrp-1阻断抗体及Nrp-1配体血管内皮生长因子165(vascular endothelial growth factor165,VEGF165)阻断抗体预孵育,体外培养72小时后流式细胞术结合Modfit软件分析肿瘤细胞增殖情况.结果:CFDA-SE标记黑色素瘤细胞生长良好,非抗体阻断组的肿瘤细胞增殖指数显著高于Nrp-1抗体阻断组[(20.67±1.16)% vs (12.67±0.28)%,P<0.01]及VEGF-165抗体阻断组[(20.67±1.16)% vs (15.15±0.83)%,P<0.01],两个抗体阻断组的增殖指数差异也非常显著 (P<0.01).结论:Nrp-1及其配体VDGF-165对小鼠黑色素瘤细胞的增殖有显著地促进作用,阻断Nrp-1产生的抑制肿瘤细胞增殖作用比阻断VEGF165更强;VEGF165/Nrp1直接作用及Nrp-1/Nrp-1直接作用也可能是肿瘤细胞增殖的重要机制. |
| 关 键 词： | 黑色素瘤 增殖 |
Effect of Neuropilin-1 in proliferation of mice melanoma cells |
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| PEI Xiang-ke,CAI Ming,ZHOU Hong-min,LIU Chang,XU Li-jun,HUANG Jian-min,CHEN Zhong-hua,SHI Bing-yi. Effect of Neuropilin-1 in proliferation of mice melanoma cells[J]. Journal of Modern Oncology, 2010, 18(2) | |
| Authors: | PEI Xiang-ke CAI Ming ZHOU Hong-min LIU Chang XU Li-jun HUANG Jian-min CHEN Zhong-hua SHI Bing-yi |
| Affiliation: | PEI Xiang-ke1,2,CAI Ming2,ZHOU Hong-min3,LIU Chang2,XU Li-jun3,HUANG Jian-min3,CHEN Zhong-hua3,SHI Bing-yi21Department of Urology,the 281st Hospital of Chinese PLA,Hebei Qinhuangdao,066100,China,2Chinese PLA Organ Transplantation Center,the 309th Hospital of Chinese PLA,Beijing 100091,3Institute of Organ Transplantation,Tongji Hospital,Tongji Medical College,Huazhong University of Science , Technology,Hebei Wuhan,430030,China. |
| Abstract: | Objective:To verificate the effect of Neuropilin-1(Nrp-1) in proliferation of mice melanoma cells.Methods:After labelled by carboxyfluorescein diacetate succinimidyl ester(CFDA-SE),melanoma cells were preincubated with Nrp-1 blocking antibody and vascular endothelial growth factor165(VEGF165) blocking antibody and were cultured in vitro for 72h.Flow cytometry and Modifit soft were used to analyse the proliferation of melanoma cells.Results: Effect of CFDA-SE labelling was excellent.Proliferation index(PI) o... |
| Keywords: | Neuropilin-1 |
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