二至天癸颗粒提高卵细胞质量与小鼠卵巢内IGF-1R mRNA表达量关系的研究

目的探讨二至天癸颗粒提高卵细胞质量的机理.方法90只小鼠分为6组,实验1组与对照1组观察排卵后优质卵数目.实验2组与对照2组观察鼠胚卵裂率.用原位杂交的方法对实验3组与对照3组鼠卵颗粒细胞IGF-1R mRNA表达量进行检验.结果实验组与对照组优质卵率分别为[（78±8）%、（71±5）%],卵裂率分别为[（88±3）%、（83±5）%]及颗粒细胞IGF-1R mRNA表达量分别为[（0.4890±0.0454）、（0.4439±0.0283）],两组比较差异有显著性（P＜0.05）.优质卵率与颗粒细胞IGF-1R mRNA表达量呈正相关.结论二至天癸颗粒提高卵细胞质量的机理可能与提高颗粒细胞IGF-1R mRNA的表达量有关.

Experimental Study on Effect of Er'zhi Tiangui Granule in Improving Quality of Oocyte and Its Correlation with Level of Insulin-like Growth Factor-1R mRNA Expression in Ovary of Mice

OBJECTIVE: To explore the mechanism of Er'zhi Tiangui Granule (ETG) in improving the quality of oocyte. METHODS: Ninety mice were randomly divided into 6 groups. The number of high-quality oocytes was comparatively observed in the 1st experimental group and the 1st control group; the embryonic cleavage rate was observed in the 2nd experimental group and the 2nd control group and the quantity of insulin-like growth factor-1R mRNA (IGF-1R mRNA) expression in ovarian granular cells was determined by using in situ hybridization in the 3rd experimental group and the 3rd control group. RESULTS: The high-quality oocytes rate, the embryonic cleavage rate and the quantity of IGF-1R mRNA expression in the three paired groups was (78 +/- 8)% vs (71 +/- 5)%, (88 +/- 3)% vs (83 +/- 5)%, 0.4890 +/- 0.0454 vs 0.4439 +/- 0.0283, respectively. The difference between the experimental groups to the respective control groups was significant (all P < 0.05), and positive correlation was shown between the high-quality oocytes rate and the quantity of IGF-1R mRNA expression. CONCLUSION: The mechanism of ETG in improving the quality of oocyte may be related with the elevation of IGF-1R mRNA level in ovarian granular cells.