| 连翘提取物对RAW264.7细胞生成NO及表达iNOS的影响 |
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| 引用本文： | 王耀,方辉,高万,侯建国,刘圆圆,赵烽.连翘提取物对RAW264.7细胞生成NO及表达iNOS的影响[J].亚太传统医药,2012,8(2):21-22. |
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| 作者姓名： | 王耀 方辉 高万 侯建国 刘圆圆 赵烽 |
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| 作者单位： | 烟台大学药学院,山东烟台,264005 |
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| 基金项目： | 国家教育部回国人员科研启动基金;山东省自然科学基金资助项目(ZR2009CM029) |
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| 摘 要： | 目的:研究连翘的抗炎作用及分子机制。方法:以LPS刺激RAW 264.7细胞,用连翘提取物进行干预,测定NO和TNF-α含量,Western blot法检测iNOS和COX-2蛋白表达水平。结果:连翘在12.5～100μg·mL-1浓度范围内可明显抑制RAW 264.7细胞释放TNF-α及NO,并可显著抑制iNOS蛋白表达。结论:连翘通过下调iNOS蛋白表达,抑制炎症介质TNF-α及NO而发挥抗炎作用。
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| 关 键 词： | 连翘 NO TNF-α iNOS COX-2 |
Effects of Forsythia Suspense Extract on NO Production and iNOS Expression in RAW 264.7 |
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| Wang Yao
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Fang Hui
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Gao Wan
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Hou Jianguo
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Liu Yuanyuan
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Zhao Feng.Effects of Forsythia Suspense Extract on NO Production and iNOS Expression in RAW 264.7[J].Asia-Pacific Traditional Medicine,2012,8(2):21-22. |
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| Authors: | Wang Yao Fang Hui Gao Wan Hou Jianguo Liu Yuanyuan Zhao Feng |
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| Institution: | *(School of Pharmacy,Yantai University,Shandong,264005,China) |
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| Abstract: | Objective:To study the anti-inflammatory mechanism of Forsythia suspense.Methods:RAW 264.7 cells were stimulated by LPS and treated with Forsythia suspense extract.NO and TNF-α levels in supernatant were detected,and the expression of iNOS and COX-2 were evaluated by Western blot.Results:Forsythia suspense strongly inhibited the production of NO,TNF-α,and the expression of iNOS.Conclusion:Forsythia suspense exert anti-inflammatory effect through iNOS pathway. |
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| Keywords: | Forsythia Suspense NO TNF-α iNOS COX-2 |
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