大鼠全脑缺血再灌注时左旋四氢巴马汀对脑内离子稳态平衡的影响

背景脑缺血再灌注可使脑内离子稳态遭到严重破坏,这种离子稳态的破坏又可加重脑缺血再灌注损伤.目的通过观察脑缺血再灌注时脑组织钙、镁、锌、钠、钾、铁含量的变化,探讨左旋四氢巴马丁在全脑缺血再灌注损伤时的作用机制.设计完全随机设计,对照实验研究.地点和材料本研究的地点为华中科技大学同济医学院附属同济医院急诊科.材料由华中科技大学同济医学院实验动物中心提供清洁级Wistar大鼠35只,雌雄不拘,体重180～200g,鼠龄4～6周.方法建立大鼠急性脑缺血再灌注损伤模型,用原子分光光度仪检测脑组织电解质含量.结果与假手术组比较脑缺血再灌注12h组钙[(218.66±11.88)μg/g,q=5.526,P＜0.01]、锌[(72.45±0.830)μg/g,q=23.240,P＜0.01]、钠[(5237.85±106.48)μg/g,q=7.956,P＜0.01]、钾[(15 421.52±264.86)μg/g,q=3.805,P＜0.05]、铁[(151.27±10.21)μg/g,q=3.392,P＜0.05]含量增高,镁[(617.71±13.91)μg/g,q=5.009,P＜0.01]含量降低;24h钙[(295.63±64.69)μg/g,q=12.251,P＜0.01]、锌[(74.44±0.47)μg/g,q=27.354,P＜0.01]含量进一步增高,镁[(587.14±10.90)μg/g,q=10.499,P＜0.01]含量进一步降低,钠[(5056.68±100.18)μg/g,q=5.269,P＜0.01]、钾[(15 116.52±631.96)μg/g,q=4.156,P＜0.05]含量仍升高,铁[(118.79±14.22)μg/g,q=2.515,P＞0.05]含量与假手术组接近.左旋四氢巴马丁在脑缺血再灌注12h和24 h均能抑制脑组织钙[(175.67±2.70)μg/g,q=3.756,P＜0.05;(190.76±4.60)μg/g,q=9.163,P＜0.01]、锌[(66.77±1.14)μg/g,q=11.758,P＜0.01;(69.94±0.98)μg/g,q=9.304,P＜0.01]、钠[(4841.94±179.00)μ.g/g,q=4.206,P＜0.05;(4 251.05±194.31)μg/g,q=3.183,P＞0.05]含量的上升,并提高脑组织镁[(706.21±25.92)μg/g,q=15.888,P＜0.01;(662.80±9.74)μg/g,q=13.585,P＜0.01]和钾[(16294.68±102.48)μg/g,q=5.274,P＜0.01;(16 577.51±152.46)μg/g,q=3.339,P＞0.05]的含量,降低铁[(86.90±2.89)μg/g,q=11.607,P＜0.01;(84.85±3.21)μg/g,q=11.795,P＜0.01)含量.结论左旋四氢巴马丁可抑制脑缺血再灌注期间脑组织钙、锌、钠含量的上升,提高镁和钾含量,降低铁含量.

Influence of L-tetrahydropalmatine on rat brain ion homeostasis in the course of cerebral ischemia reperfusion

BACKGROUND: Cerebral ischemia-reperfusion leads to disruption of brainion homeostasis that aggravates ischemia-reperfusion injury.OBJECTIVE: To investigate the action mechanism of L-tetrahydropalmatine(L-THP) in cerebral ischemia-reperfusion injury by observing the changes inthe contents of calcium, magnesium, zinc, sodium, potassium and iron in thebrain tissues.DESIGN: Completely randomized controlled study.SETTING and MATERIALS: This study was carried out in the EmergencyDepartment, Tongji Hospital, Huazhong University of Science and Technolo-gy. Thirty-five Wistar rats of clean grade weighing 180 - 200 g and aged 4 -6 weeks old were used.METHODS: Rat models of cerebral ischemia-reperfusion injury were estab-lished to determine the electrolyte contents using an atomic absorptionspectrophotometer ( AAS ).RESULTS: Compared with the sham operation group, the cerebral ischemia-reperfusion group, at 12 hours, had elevated levels of brain calcium[ (218.66 ±11.88) μg/g, q=5.526, P ＜0.01], zinc[(72.45±0.83) μg/g, q=23. 240, P ＜ 0.01 }, sodium[ (5 237.85 ± 106.48) μg/g, q = 7. 956, P ＜0.01], potassium[(15 421.52 ±264. 86) μg/g, q =3. 805, P ＜0.05] andiron[ (151.27 ± 10. 21 ) μg/g, q = 3. 392, P ＜ 0.05], but with lowered mag-nesium content; at 24 hours after the injury, further increases occurred in thelevels of calcium[(295. 63 ±64. 69) μg/g, q=12.251, P ＜0.01], zinc[(74.44±0.47) μg/g, q=27.354, P ＜0.01], sodium[ (5 056.68±100.18) μg/g, q=5.269, P ＜ 0.01] and potassium[(15 116.52±631.96) μg/g, q =4. 156, P ＜ 0. 05], while the content of magnesium[(587.14±10.90) μg/g, q=10.499, P ＜0.01] decreased, and thecontent of iron[(118. 79±14.22) μg/g, q=2.515, P＞ 0.05] wassimilar to that in the sham operation group. At both 12 and 24 hours after thereperfusion, L-THP inhibited the increase in the contents of calcium[ (175.67 ± 2.70) μg/g, q = 3. 756, P ＜ 0. 05; (190. 76 ± 4.60) μg/g,q=9.163, P ＜0.01], zinc[(66.77±1. 14) μg/g, q=11.758, P ＜0.01; (69.94 ± 0.98) μg/g, q = 9. 304, P ＜0.01] and sodium[ (4 841.94 ±179.00) μg/g, q=4.206, P ＜0.05; (4251.05±-194.31) μg/g, q=3. 183, P ＞ 0. 05], increased the contents of magnesium [ (706.21 ±-25.92) μg/g, q=15.888, P ＜ 0.01; (662.80±9.74) μg/g, q=13.585, P ＜0.01] and potassium[(16 294.68±102.48) μg/g, q=5. 274, P ＜ 0.01; (16 577.51 ± 152.46) μg/g, q = 3. 339, P ＞ 0.05], anddecreased the contents of iron[(86.90±2.89) μg/g, q=11.607, P ＜0.01;(84.85±3.21) μg/g, q=11.795, P ＜0.01].CONCLUSION: L-THP can inhibit the increase of calcium, zinc and sodiumcontents, increase the contents of magnesium and potassium, and decrease thecontent of iron in rat brain tissues during cerebral ischemia-reperfusion.`