| 反相高效液相色谱法测定血浆中卡维地洛的浓度 |
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| 引用本文： | 扈本荃,廉江平,张鹏,王弼时.反相高效液相色谱法测定血浆中卡维地洛的浓度[J].解放军药学学报,2007,23(1):46-48. |
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| 作者姓名： | 扈本荃 廉江平 张鹏 王弼时 |
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| 作者单位： | 1. 第四军医大学唐都医院,药剂科,陕西,西安,710038
2. 陕西省人民医院,陕西,西安,710068 |
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| 摘 要： | 目的建立反相高效液相色谱法测定血浆中卡维地洛的浓度。方法采用反相高效液相色谱法测定血浆中卡维地洛的浓度，选用Shim-pack VP-ODS柱（150mm×4．6mm，5μm），甲醇：水（60：40）]-乙腈（88：12）为流动相，流速：1ml／min，检测波长：280nm。结果卡维地洛血药浓度的线性范围为25～2000ng·ml^-1（r=0．9999），提取回收率70．84％，方法回收率102．05％,日内、日间RSD分别为1．91％、5．58％。结论该方法准确、灵敏，适于卡维地洛药代动力学研究。
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| 关 键 词： | 反相高效液相色谱法 卡维地洛 血药浓度 |
| 文章编号： | 1008-9926（2007）01-0046-03 |
| 收稿时间： | 2005-08-15 |
| 修稿时间： | 2006-10-10 |
RP-HPLC Determination of Carvedilol in Human Plasma |
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| HU Ben-Quan,LIAN Jiang-Ping,ZHANG Peng,WANG Bi-Shi.RP-HPLC Determination of Carvedilol in Human Plasma[J].Pharmaceutical Journal of Chinese People's Liberation Army,2007,23(1):46-48. |
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| Authors: | HU Ben-Quan LIAN Jiang-Ping ZHANG Peng WANG Bi-Shi |
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| Institution: | HU Ben-Quan, LIAN Jiang-Ping,ZHANG Peng, WANG Bi-Shi (1.Department of Pharmacy, Tangdu Hospital, Fourth Military Medical University, Xi＇an 710038,Shaanxi China;2.Department of Pharmacy, The People＇s Hospital of Shaanxi Province, Xi＇an 710068, Shaanxi China ) |
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| Abstract: | Aim An RP-HPLC method was developed for detemination of Carvedilol in human plasma. Methods Carvedilol was extracted from plasma and the applied column was Shim-pack VP-ODS （150mm ×4.6mm,5μm）. The mobile phase consisted of methanol and water（60： 40）- acetonitrile（88： 12）. Carvedilol was detected by UV absorption at 280nm. Results The standard curve was liner in the range of 25 - 2 000ng.ml^-1（ r =0.999 9） ;the extracted recovery of Carvedilol was 70. 84% ;the recovery of the method was 102.05% ;and the RSDs of intra-day and inter-day were 1.91% and 5.58%. Conclusion the method is sensitive and accurate. |
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| Keywords: | RP-HPLC carvedilol plasma concentration |
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