半枝莲抑制人肝癌QGY-7701细胞增殖研究

目的 探讨半枝莲抑制人肝癌QGY-7701细胞的初步机制。方法 MTT法检测半枝莲作用后QGY-7701细胞增殖的改变，光、电镜观察其形态变化，流式细胞仪检测其凋亡细胞比例、细胞周期的变化及凋亡相关基因Bcl-2、Bax、Fas表达。结果 半枝莲能抑制QGY-7701细胞的增殖，作用72h后，细胞呈现凋亡早期形态；凋亡细胞增多：G0／G1期细胞减少，G2／M期细胞增多；Bax表达增强，Bcl-2表达减少，Fas表达变化不明显。结论 半枝莲可抑制QGY-7701细胞增殖，可能与通过激活Bcl-2基因家族抑癌基因、诱导细胞凋亡、阻滞细胞周期有关。

Inhibition activity of Scutellariae barbata extracts against human hepatocellular carcinoma cells

Objective To ,investigate the inhibition effect of Scutellariae barbara extracts on the proliferation of human hepatocellular carcinoma cell line QGY-7701. Methods The inhibition activity of the extracts against cell line QGY-7701 was estimated by MTT assay. Morphologic changes of the cells were observed under light microscope and electronic microscope. Cell cycle distribution, cell apoptosis and expressions of apoptosis-associated genes as Bcl-2,Bax and Fas were analyzed by flow cytomefry. Results Extracts of Scutellariae barbara could inhibit the proliferation of QGY-7701. When treated with the extracts for 72 h, cells at the early stage of apoptosis showed morphologic changes, apoptotic cells increased, cells at G0/G1 phase decreased and those at G2/M phase increased, the expression of Bax significantly up-regulated and that of Bcl-2 down-regulated w/th no change in Fas gene. Conclusion The extracts of Scutellariae barbara inhibit the proliferation of QGY-7701, which may relate to the activation of anti-oncogene Bcl-2, induction of cell apoptosis and inhibition of G to S phase cell cycle progress.