固相萃取-HPLC法同时测定妇科千金片中穿心莲内酯和脱水穿心莲内酯的含量

目的：建立妇科千金片中穿心莲内酯和脱水穿心莲内酯含量的测定方法。方法：色谱柱：XTerra RP C19（150mm×3．9mm，5μm），流动相：甲醇-水（45：55），采用固相萃取（SPE）-HPLC法测定。结果：穿心莲内酯线性范围为16．98～169．80μg·ml^-1（r=0．9999），平均回收率为98．74％，RSD=1．91％（n=6）；脱水穿心莲内酯线性范围为31．78～317．80μg·ml^-1（r=0．9999），平均回收率为99．04％，RSD=1．54％（n=6）。结论：本法操作简单、结果准确，可用于妇科千金片的质量控制。

Simultaneous Determination of Andrographolide and Dehyandrographolide in Fukeqianjin Tablets by SPE-HPLC

Objective： To establish a method to determine the contents of andrographolide and dehyandrographolide in Fukeqianjin tablets. Method： SPE- HPLC was conducted on a XTerra RPC18 column（ 150mm × 3.9mm,5μm）, mobile phase was consisted of methanol-water （45：55 ）. Result： The linear range of andrographolide was 16.98 - 169.80μg·ml^-1 （ r = 0.999 9 ） , and the average recovery was 98.74% （RSD = 1.91%, n = 6） ;the linear range of dehydroandrographolide was 31.78 -317.80μg·ml^- 1 （r = 0. 999 9 ）, and the average recovery was 99.04% （RSD = 1.54%, n = 6）. Conclusion： This method is simple and feasible for the quality control of Fukeqianjin tablets.