| 培养温度、时间及db-cAMP对骨髓细胞~3H-TdR参入的影响 |
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| 引用本文： | 蒋亦寿,王天宇,杨丽君,郑如兰. 培养温度、时间及db-cAMP对骨髓细胞~3H-TdR参入的影响[J]. 南京医科大学学报(自然科学版), 1984, 0(4) |
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| 作者姓名： | 蒋亦寿 王天宇 杨丽君 郑如兰 |
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| 作者单位： | 南京医学院基础同位素应用实验室(蒋亦寿，王天宇，杨丽君)，南京医学院基础同位素应用实验室(郑如兰) |
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| 摘 要： | 本文以~3H-TdR参入技术探讨了几种因素对短期离体培养的小鼠骨髓造血细胞增殖活性的影响。结果表明:温度和时间的影响是十分明显的。为减少骨髓细胞原有的~3H_TdR参入活性的变化,骨髓样品应放在4℃下。db-cAMP的作用决定于浓度。10M~(-8)时,db-cAMP具有刺激效应。大于或小于该浓度,它显示抑制作用。
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| 关 键 词： | 骨髓细胞培养 db-cAMP ~3H-TdR参入术 |
EFFECTS OF THE CULTURAL TEMPERATURE TIME AND db-cAMP OF THE CULTURE ON THE ~3H-TdR INCORPO- RATION OF THE BONE MARROW CELLS |
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| Jiang Yishou et al.. EFFECTS OF THE CULTURAL TEMPERATURE TIME AND db-cAMP OF THE CULTURE ON THE ~3H-TdR INCORPO- RATION OF THE BONE MARROW CELLS[J]. Acta Universitatis Medicinalis Nanjing, 1984, 0(4) |
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| Authors: | Jiang Yishou et al. |
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| Affiliation: | Jiang Yishou et al. |
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| Abstract: | A technique using ~3H-TdR incorpora-tion was developed to investigate the in-fluence of some factors on the prolifera-tion of the mouse bone marrow hematop-oietic cells in short-term culture. The results indicated that the effectsof temperature and time were very ob-vious. In order to minimize the change ofthe 3H-TdR incorporated activity of thehematopoietic cells, the sample should bekept at 4 c. The effects of the exogenousdb-cAMP depend on its concentration. db-cAMP 10~(-8)M had an irritant effect.Otherwise, either at higher or lower conc-entration, it presented an inhibited effect. |
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| Keywords: | Bone marrow cell ~3H-TdR incorporation Culture temperature Time db-cAMP |
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