非小细胞肺癌中p16基因表达的意义

 目的　检测人NSCLC中p16基因蛋白表达与NSCLC的临床病理类型及预后的关系。方法　用S—P免疫组化法对80例NSCLC及40例肺部良性病变的石蜡标本中p16蛋白进行对比检测。结果　(1)NSCLC中p16蛋白阳性率为55%(44/80)，良性病变的阳性率为92.5%(37/40)，两者存在显著性意义(P<0.005)。(2)NSCLC中p16蛋白表达下降的程度随病期进展而明显，提示对预后判断有意义。(3)p16蛋白表达与NSCLC的具体病理亚型无明确关系。结论　S—P法检测分析p16基因的表达产物简便易行，对预后判断有意义。

The Significance of p16 Gene Expression in Human Non-small Cell Lung Cancer

Objective To examine p16 gene expression in human non-small cell lung cancer (NSCLC) and to took for correlations among P16 expression and clinicopathologic features and prognosis.Methods With S P immunohistochemistry method,investigated p16 protein expression in 80 patients with primary NSCLC and 40 with lung benign diseases (as control) with archival paraffin specimens.Results The positive rate of p16 expression in NSCLC and lung benign diseases were 55% (40/80) and 92 5% (37/40),respectively.There was significant difference between the two groups.( P <0 005).The positive rate of p16 expression was related to clinical stage of NSCLC.lower p16 expression in NSCLC was seen with advanced pathological stage of disease.The positive rate of p16 expression in NSCLC had no correlation to its histopathological subtype.Conclusion Immunohistochemical analysis of the p16 gene/MTS 1 products is feasible.These results suggest that p16 /MTS 1 down regulation expression plays an important role in tumorigenesis or tumor progression of NSCLC.Aberrant expression of p16/MTS 1 gene may assess the prognosis in NSCLC.