| 单克隆抗体间接免疫荧光试验检测生殖器单纯疱疹病毒感染 |
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| 引用本文： | 刘军连,徐志凯,喻启桂,王红英,郑燕华,吕世超,张建中. 单克隆抗体间接免疫荧光试验检测生殖器单纯疱疹病毒感染[J]. 医学争鸣, 2004, 25(8): 728-729 |
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| 作者姓名： | 刘军连 徐志凯 喻启桂 王红英 郑燕华 吕世超 张建中 |
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| 作者单位： | 解放军第306医院皮肤科,北京100101;第四军医大学,基础部微生物学教研室,陕西,西安,710033;第四军医大学,西京医院妇产科,陕西,西安,710033 |
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| 摘 要： | 目的:评价间接免疫荧光试验 (IFA)在生殖器疱疹(GH)诊断中的应用价值.方法:采用以单纯疱疹病毒(HSV)型共同性单克隆抗体为夹心的IFA方法,检测了120例临床诊断为GH患者皮疹中的HSV,并与病毒培养法进行比较.结果:IFA检测HSV的总阳性率为85.8%,高于病毒培养法的阳性率(70.8%,χ2=12.04,P<0.01).两种方法检测GH患者皮肤水疱内的HSV阳性率分别为93.3%和90.0%,无明显差异(χ2=1.96,P>0.05);而检测糜烂和结痂性皮疹内的HSV时,IFA 的阳性率分别为92.6%和69.4%,均分别高于病毒培养法(75.9%,χ2=5.82,P<0.05;47.2%,χ2=14.17,P<0.01).结论:IFA 法具有简单、快速、敏感性高的优点,适用于检测GH患者皮疹内HSV,有临床实用价值.
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| 关 键 词： | 单纯病毒属 生殖器疱疹 抗体 单克隆 荧光抗体技术 间接检测 |
| 文章编号： | 1000-2790(2004)08-0728-02 |
| 修稿时间： | 2004-01-28 |
Indirect immunofluorescence assay with monoclonal antibodies in detecting genital herpes simplex virus infection |
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| Abstract: | AIM: To evaluate the application of indirect immunofluorescence assay (IFA) in the diagnosis of genital herpes. METHODS: Both viral culture and IFA mediated by group shared herpes simplex virus (HSV) glucoprotein monoclonal antibodies were adopted in detecting 120 patients with clinically diagnosed genital herpes. RESULTS: The HSV positivity rate detected by IFA was significantly higher than that by viral culture (85.8% vs 70.8%, P < 0.01). Different from those in detecting HSV from erosion and crust eruption, the positivity rates in detecting HSV from blister by the two methods were of no statistical significance ( P >0.05). CONCLUSION: IFA is a cheap, rapid and sensitive method for HSV detection and can be used in the diagnosis of genital herpes. |
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| Keywords: | simplex virus herpes genitalis antibody monoclonal immunofluorescence assay antibody technique indirect detection |
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