A research for the relationship between human papillomavirus and human uterine cervical carcinoma |
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| Authors: | Jingyi Si Kun Lee Ricai Han Wei Zhang Bingbing Tan Guoxing Song Shide Liu Lianfong Chen Weiming Zhao Liping Jia Yongyan Mai Yi Zeng Yinan Zhou Yuezhu Wang Jian Ling Yu Sun Xiangjin Meng Zhangfong Yu Liming Pu |
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| Affiliation: | (1) Department of Biophysics, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and the Peking Union Medical College, 5 Dong Dan San Tiao, 100005 Beijing, China;(2) Department of Gynecology and Obstetrics, The First Teaching Hospital, Beijing Medical University, 100034 Beijing, China;(3) Department of Tumor Viruses, Institute of Virology, Chinese Academy of Preventive Medicine, 100 Yin Xin Jie, 100052 Beijing, China;(4) Department of Pathology, Gynecology and Obstetrics, The First Teaching Hospital, Xinjiang Medical College, Urumchi, 830054 Xinjiang Uighur Autonomous Region, China;(5) Institute of Virology, Hubei Medical College, Wuhan, 430071 Hubei Province, China;(6) Department of Epidemiology, Faculty of Public Health, Harbin Medical University, Harbin, 150001 Heilongjiang Province, China |
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| Abstract: | Summary Biopsies from 318 cases with squamous cell carcinoma of the uterine cervix, 48 with cervical and vulvar condylomata, 14 with cervical intraepithelial neoplasia (CIN), 34 with chronic cervicitis and 24 with normal cervical epithelium were collected from different geographic regions with different cervical cancer mortalities. The DNA · DNA dot-blot and Southern blot hybridization results show that there is a close relationship between HPV-16 and the uterine cervical squamous cell carcinoma in China. One very interesting observation is that the finding of HPV-16-homologous DNA differs significantly among five geographic regions, and corresponds with the mortalities from cervical cancer of these five regions. HPV-11 was found mainly in benign lesions. The rate of detection of HPV-16 in Chinese women increased from 8.3% in normal cervical epithelium to 20% in chronic cervicitis, 28% in cervical condyloma, 50% in CIN and 60.4% in cervical cancer. It is suggested that HPV-16 infection may be an etiological factor in the development of human cervical carcinoma. From the results of Southern blot hybridization, it appeared that HPV-16 DNA had been integrated into the genome of the host cell in cervical cancer. Whereas the HPV-16 DNA sequence was only present as an episome in normal cervical epithelium and cervical benign lesions. The rate of occurence of E6-E7 genes is the highest (88.9%) compared with that of other subgenomic fragments of HPV-16 in specimens of human cervical cancer in China. This implies that E6 and E7 may be the oncogenic genes of HPV-16 and play an important role in the carcinogenesis of human cervical epithelial cells. The amplification and rearrangement of the c-myc protooncogene are closely associated with the occurrence of cervical cancer. The results presented here revealed that the activated c-myc oncogene may cooperate with HPV-16 in the carcinogenic processes.Abbreviations HPV Human papillomavirus - CIN cervical intraepithelial neoplasia - SSC standard saline citrate |
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| Keywords: | Uterine cervical carcinoma Human papillo-mavirus Molecular epidemiology Carcinogenesis Viral oncogene |
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