| 一种快速稳定的人精子核双色荧光原位杂交技术的建立 |
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| 引用本文： | 刘浩,张昌军,王华,罗清炳,刘海湘.一种快速稳定的人精子核双色荧光原位杂交技术的建立[J].武汉大学学报(医学版),2005,26(2):207-209,i003. |
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| 作者姓名： | 刘浩 张昌军 王华 罗清炳 刘海湘 |
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| 作者单位： | 湖北省十堰市人民医院生殖医学研究中心,十堰,442000;湖北省十堰市人民医院生殖医学研究中心,十堰,442000;湖北省十堰市人民医院生殖医学研究中心,十堰,442000;湖北省十堰市人民医院生殖医学研究中心,十堰,442000;湖北省十堰市人民医院生殖医学研究中心,十堰,442000 |
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| 摘 要： | 目的:建立快速稳定的人精子核双色荧光原位杂交的实验方法。方法:采用双色荧光原位杂交(fluores cence in situ hybridization, FISH )方法取精子标本用EDTA/PBS液处理,再用二硫苏糖醇(DTT)使精子去凝集,甲醇/冰醋酸固定后制片,变性后与双色荧光直接标记(CEP X、Y)探针杂交。结果:在荧光显微镜下可见标本背景清晰,精子头部染成蓝色,边界清楚,头部有1个绿色荧光信号的精子为 X精子,有 1 个红色荧光信号的精子为 Y精子。精子头部有2个或2个以上信号的精子为染色体数目异常精子。结论:本方法具有快速、稳定、荧光信号强、易分辨等优点,可用于人性染色体非整倍体率的分析研究。
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| 关 键 词： | 荧光原位杂交 人精子染色体 非整倍体 |
| 文章编号： | 1671-8852(2005)02-0207-03 |
Establishment of An Effective Approach to Dual-color Fluorescence in situ Hybridization of Human Sperm Interphase Nucleus |
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| Abstract: | Objective: To establish an effective approach to dual-color fluorescence in situ hybridization of human sperm interphase nucleus.Methods: Dual-color fluorescence in situ hybridization (FISH) was performed on human sperm interphase nucleus using centromeric probes for chromosome X and Y. Results: It was seen under the fluorescence microscope that the sperm showed with one green hybridization signal on the head was X chromosomal sperm, while with one red hybridization signal was Y chromosomal sperm, and those with 2 or >2 fluorescence hybridization signals on the sperm head were abnormal counts for chromosomal sperm. Conclusion: The hybridization signals are visible and distinguishable, and this method is effective. It could be used for analyzing aneupoidy of human sperm chromosome. |
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| Keywords: | Fluorescence in situ Hybridization Human Sperm Chromosome Aneupoidy |
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