| Lck对肾小管上皮细胞的IL-12信号传递功能研究 |
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| 引用本文： | 李清刚,李志坚,李幼姬,王俭勤.Lck对肾小管上皮细胞的IL-12信号传递功能研究[J].中华微生物学和免疫学杂志,2001,21(6):590-593. |
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| 作者姓名： | 李清刚 李志坚 李幼姬 王俭勤 |
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| 作者单位： | 1. 首都医科大学附属北京友谊医院肾内科
2. 中山医科大学附属第一医院肾内科卫生部 |
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| 摘 要： | 目的 研究lck途径激活后对小鼠肾小管上皮细胞的信号传递功能的影响。方法 应用IL-12、IL-2刺激小管皮细胞，通过原位杂交检测lckmRNA在小管上皮细胞的表达；采用lck抑制剂PP1，阻抑小管上皮细胞，通过原位杂交检测lckmRNA在小管上皮细胞的表达；采用lck抑制剂PP1，阻抑小管上皮细胞的lck激酶，通过免疫印迹和放射自显影方法检测lck对小鼠上皮细胞的信号传递功能的影响。结果 lck激酶激活可调控小管上皮细胞的lck/c-Jun信号传递途径；小管上皮细胞中lck途径激活介导的细胞炎症作用与IL-12的c-Jun表达作用有关。结论 lck在IL-12的信号传递中发挥重要的作用。
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| 关 键 词： | 白细胞介素12 肾小管上皮细胞 信号传递 肾小管损伤 lck |
| 修稿时间： | 2000年12月4日 |
Investigation of lck on IL-12 signal transduction in tubular epithelial cells |
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| LI Qinggang ,LI Zhijian,LI Youji,et al..Investigation of lck on IL-12 signal transduction in tubular epithelial cells[J].Chinese Journal of Microbiology and Immunology,2001,21(6):590-593. |
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| Authors: | LI Qinggang LI Zhijian LI Youji |
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| Institution: | LI Qinggang *,LI Zhijian,LI Youji,et al. *Department of Nephrology,Beijing Friendship Hospital,Beijing 100050,P. R. China [ |
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| Abstract: | Objective To investigate the effect of lck pathway activation on IL 12 signal transduction of the tubular epithelial cells. Methods Murine tubular epithelial cells(TEC) were stimulated with IL 12 or IL 2, expression of lck mRNA was determined by in situ hybridyzation. When TEC was cultivated with lck specific inhibitor PP1 to inhibit lck activity, effect of lck on IL 12 signal transduction of TEC was measured by autoradiography and immunoblot assay, respectively. Results lck may regulate the lck/c jun signaling pathways in TEC. Further, inflammation of TEC mediated by the activation of lck pathway is related to the expression of c Jun promoted by IL 12. Conclusion lck play an important role in the signal transdution of IL 12 in tubular epithelial cells. |
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| Keywords: | Interleukin 12 Tubular epithelial cell Signal transduction lck |
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