大鼠坐骨神经损伤后显微及超微结构的三维重建

利用大鼠坐骨神经损伤后再生神经组织连续切片重建其显微及超微结构的三维图像。用硅胶管桥接90只大鼠左侧坐骨神经长6mm缺损，术后7、15、30、60、90天取坐骨神经行光、电镜检查，经显微摄像和扫描仪采集输入微机进行图像配准和分割，采用直接体素绘制模型实现三维重建和显示。结果显示，利用光镜和电镜图像完成了坐骨神经损伤后变性、再生过程中神经纤维及附属结构的形态变化的三维图像重建。重建结果能直观、形象地揭示大鼠再生坐骨神经纤维与正常神经纤维结构形态的差异，可作为周围神经损伤研究的一种新手段。

3-D (THREE DIMENSIONAL) RECONSTRUCTION OF MICROSTRUCTURE AND ULTRASTRUCTURE OF THE SCIAT-IC NERVE OF RAT AFTER INJURY

To reconstruct 3 D images of microstructure and ultrastructure of regenerated sciatic nerve of rats with serial cross sections. The 6 mm defects of the sciatic nerve of 90 Wistar rats were bridge connected with silicone tube and were sampled at 3, 7, 15, 30, 60, 90 days respectively after injury. The images of optical and electronic microscope were taken by micro photograph system and scanner, then they were input into the computer, by which the image registration and segmentation were completed. The direct volume rendering model was used to realize the 3 D reconstruction and display. The results showed the morphological changes in degenerated and regenerated sciatic nerve fibres and their appendicular structures could be observed with the 3 D images. It is suggested that the reconstructing results could reveal the differences between the regenerated and normal sciatic nerve fibres of rats intuitively and visually, so it could be used as a new method in the study of peripheral nerve injury.