| ELF3多克隆抗体的制备及其免疫定位 |
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| 引用本文： | 汪志军,常莹,吴伟,姚津剑,何星星,林菊生,宋宇虎. ELF3多克隆抗体的制备及其免疫定位[J]. 华中科技大学学报(医学版), 2010, 39(1). DOI: 10.3870/j.issn.1672-0741.2010.01.002 |
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| 作者姓名： | 汪志军 常莹 吴伟 姚津剑 何星星 林菊生 宋宇虎 |
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| 作者单位： | 1. 华中科技大学同济医学院附属同济医院肝病研究所,武汉,430030
2. 华中科技大学同济医学院附属协和医院消化内科,武汉,430022 |
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| 基金项目： | 国家自然科学基金资助项目 |
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| 摘 要： | 目的 制备并鉴定兔抗小鼠胚肝血影蛋白3(embryonic liver fodrin 3,ELF3)的多克隆抗体,并探讨其在部分小鼠组织中的免疫定位.方法 合成ELF3氨基端特异性多肽片段,连接载体蛋白钥孔戚血蓝素(keyhole limpet hemocyanin,KLH),免疫新西兰大白兔,制备多克隆抗体,用ELISA方法 检测抗体效价,Western blot和免疫组织化学方法 检测抗体特异性及在小鼠组织中的表达情况.结果合成ELF3特异性多肽,并连接KLH后免疫实验兔,制备得到兔抗小鼠ELF3多克隆抗体,ELISA及Western blot检测结果显示此抗体效价及特异性高;Western blot和免疫组织化学检测结果显示ELF3在心、肝、肾组织中表达,尤其是细胞膜上表达明显.结论 本实验成功制备ELF3多克隆抗体,并证实ELF3在小鼠心、肝、肾组织细胞膜表达明显,这将为ELF3功能研究提供有力的工具.
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| 关 键 词： | 胚肝血影蛋白3 抗体制备 免疫定位 |
Preparation of Rabbit Polyclonal Antibody against ELF3 and Its Immunolocalization in Mice |
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| Wang Zhijun,Chang Ying,Wu Wei,Yao Jinjian,He Xianxian,Lin Jusheng,Song Xuhu. Preparation of Rabbit Polyclonal Antibody against ELF3 and Its Immunolocalization in Mice[J]. Journal of Huazhong University of Science and Technology(Health Sciences), 2010, 39(1). DOI: 10.3870/j.issn.1672-0741.2010.01.002 |
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| Authors: | Wang Zhijun Chang Ying Wu Wei Yao Jinjian He Xianxian Lin Jusheng Song Xuhu |
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| Affiliation: | Wang Zhijun,Chang Ying,Wu Wei et al Institute of Liver Diseases,Tongji Hospital,Tongji Medical College,Huazhong University of Science , Technology,Wuhan 430030,China |
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| Abstract: | Objective To prepare and identify rabbit polyclonal antibody against embryonic liver fordrin 3(ELF3),and investigate the distribution of ELF3 in mice tissue.Methods ELF3 specific N-terminal peptide was synthesized,and conjugated to Keyhole limpet hemocyanin(KLH)as immunogen.The ELF3-KLH complex was injected into rabbits subcutaneously,and then ELF3 antibody was purified using affinity chromatography.The titer of the antibody was evaluated by ELISA.The specificity of antibody against ELF3 and immunolocalizat... |
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| Keywords: | embryonic liver fodrin 3 antibody preparation immunolocalization |
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