| 牛磺酸抑制过氧化氢诱发牛晶状体上皮细胞凋亡的实验研究 |
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| 引用本文： | Chen F,Chen C. 牛磺酸抑制过氧化氢诱发牛晶状体上皮细胞凋亡的实验研究[J]. 中华眼科杂志, 2000, 36(4): 272-274,I017 |
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| 作者姓名： | Chen F Chen C |
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| 作者单位： | 北京市眼科研究所(陈凤华!100005),首都医科大学!100054(陈翠真) |
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| 基金项目： | 国家自然科学基金!( 3 9770 786) |
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| 摘 要： | 观察牛磺酸对对过氧化氢（Ｈ２Ｏ２）诱发牛晶状体上皮细胞凋亡的抑制作用。方法采用牛晶状体器官培养及ＤＮＡ缺口末端原位标记法检测牛磺酸对Ｈ２Ｏ２诱发的牛晶状体上皮凋亡细胞数量的影响,并进行相关比较及统计学分析。结果晶状体上皮细胞凋亡发生于晶状体混浊之前;牛磺酸可明显抑制Ｈ２Ｏ２所致的牛晶状体上皮细胞凋亡。
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| 关 键 词： | 牛磺酸 过氧化氢 晶状体 上皮细胞 白内障 |
An experimental research of taurine on H2O2-induced bovine lens epithelial cell apoptosis |
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| Chen F,Chen C. An experimental research of taurine on H2O2-induced bovine lens epithelial cell apoptosis[J]. Chinese Journal of Ophthalmology, 2000, 36(4): 272-274,I017 |
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| Authors: | Chen F Chen C |
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| Affiliation: | Beijing Institute of Ophthalmology, Beijing 100005, China. |
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| Abstract: | Objective To study the inhibitory role of taurine on H 2O 2 induced bovine lens epithelial cell apoptosis Methods By terminal deoxyribonucleotide transferase mediated dUTP nick end labeling (TUNEL) detection and bovine lens organ culture, we examined the affect of taurine on the number of H 2O 2 induced bovine lens epithelial cells with apoptosis, the related comparisons and statistic analyses were carried out Results (1) Lens epithelial cell apoptosis began before lens opaqueness (lens became opaque after 6 hours of incubation, while the lens epithelial cell apoptosis was detected after 3 hours of incubation); (2) Taurine could apparently inhibit H 2O 2 induced bovine lens epithelial cell apoptosis Conclusion Taurine has an inhibitory role on H 2O 2 induced bovine lens epithelial cell apoptosis and can delay and ameliorate the occurrence and development of cataract |
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| Keywords: | Taurine Hydrogen peroxide Lens Epithelial cells Apoptosis |
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