散血草水提物抗胃溃疡的实验研究

目的观察散血草水提物对胃溃疡小鼠的影响,为临床应用提供实验依据。方法采用利血平诱导制作胃溃疡动物模型。实验小鼠随机分为正常组、模型组、散血草低剂量组、散血草高剂量组、雷尼替丁组,各给药组给予相应药物灌胃,正常组和模型组给予等量生理盐水,连续4周。测定胃溃疡面积、胃液量和胃液酸度,酶联免疫法检测血清中相关因子水平,RT-PCR法检测胃组织诱导型一氧化氮合酶(iNOS)、环氧化酶-2(COX-2)基因表达。结果与模型组比较,散血草低、高剂量组胃溃疡面积明显减少,血清中生长抑素和血管活性肠肽水平升高,胃动素和P物质水平降低(P0.05,P0.01);散血草低、高剂量组白细胞介素(IL)-6、IL-12、肿瘤坏死因子-α、γ-干扰素水平及iNOS、COX-2基因表达水平降低(P0.05,P0.01)。结论散血草具有较好的抗胃溃疡作用,散血草高剂量组表现更优。

Experimental Study on Anti-Gastric Ulcer Effect of Aqueous Extractive of Clarke Boea Herb

Objective To observe effects of aqueous extractive of Clarke boea herb on mice with gastric ulcer, and provide basis for clinical application.Methods Gastric ulcer model was induced by reserpine. The mice were divided into normal group, model group, ranitidine group, low and high dose of Clarke boea herb groups. Mice in treatment groups were given corresponding drugs by gavage, and the normal group and control group were given the same amount of saline for 4 weeks. The area of gastric ulcer, serum proinflammatory cytokines gastric juice volume, gastric juice acidity, iNOS and COX-2 gene expressions were detected.Results Compared with control group, the gastric ulcer area in low and high dose of Clarke boea herb groups decreased significantly. Clarke boea herb increased the SS and VIP, decreased the MOT and SP serum levels （P〈0.05,P〈0.01）. Clarke boea herb reduced the levels of serum proinflammatory cytokines of IL-6, IL-12, TNF-α and IFN-γ（P〈0.05,P〈0.01）. Clarke boea herb also decreased the genetic expressions of iNOS and COX-2 （P〈0.05,P〈0.01）.ConclusionClarke boea herb has good gastric ulcer preventive effects, and the high dose of Clarke boea herb group showed the best efficiency.