| 考来烯胺对胆汁酸合成的基因调节作用 |
| |
| 引用本文： | 邱冬妮,孙旭,钟良,毛奇琦,孙大裕. 考来烯胺对胆汁酸合成的基因调节作用[J]. 肝脏, 2008, 13(5): 390-392 |
| |
| 作者姓名： | 邱冬妮 孙旭 钟良 毛奇琦 孙大裕 |
| |
| 作者单位： | 复旦大学附属华山医院消化内科,上海,200040 |
| |
| 摘 要： | 目的研究考来烯胺促进肝内胆汁酸合成的基因调节机制。方法20只新西兰白兔分为考来烯胺组（考来烯胺每天1g/kg,灌胃2周）和对照组,每组10只。测定两组2周后的血清胆固醇水平、总胆汁酸水平、肝组织胆固醇7α-羟化酶（CYP7A1）活性及其mRNA、法尼酯衍生物X受体（FXR）的靶基因短异源二聚体伴侣受体（SHP）mRNA和胆盐输出泵（BSEP）mRNA、低密度脂蛋白受体（LDL-R）mRNA表达。结果考来烯胺组血清胆固醇水平较对照组下降10.25%,而血清总胆汁酸水平无明显改变;肝脏CYP7A1活性及其mRNA表达较对照组显著升高（P〈0.05）,FXR靶基因SHPmRNA和BSEPmRNA表达较对照组显著降低（P〈0.05）,LDL-RmRNA表达较对照组显著升高（P〈0.05）。结论考来烯胺通过抑制回肠胆汁酸重吸收,减少回流入肝脏的胆汁酸（FXR配体）,从而抑制肝内FXR,激活CYP7A1,促进肝内胆固醇转化为胆汁酸,继而维持体内胆汁酸池稳定,降低血清胆固醇水平。
|
| 关 键 词： | 胆汁酸 考来烯胺 法尼酯衍生物X受体 胆固醇7α-羟化酶 |
Moleculer mechanisms of cholestyramine in promoting bile acid synthesis |
| |
| QIU Dong-ni,SUN Xu,ZHONG Liang,MAO Qi-qi,Liu Yi,SUN Da-yu. Moleculer mechanisms of cholestyramine in promoting bile acid synthesis[J]. Chinese Hepatology, 2008, 13(5): 390-392 |
| |
| Authors: | QIU Dong-ni SUN Xu ZHONG Liang MAO Qi-qi Liu Yi SUN Da-yu |
| |
| Affiliation: | QIU Dong-ni,SUN Xu,ZHONG Liang,MAO Qi-qi,Liu Yi,SUN Da-yu. Department of Gastroenterology,Huashan Hospital of Fudan University,Shanghai 200040,China |
| |
| Abstract: | Objective To study the possible mechanisms of cholestyramine promoting bile acid synthesis in vitro.Methods Twenty New Zealand white rabbits were divided into cholestyramine group (n=10) and control group (n=10). Each rabbit in treatment group was administered with cholestyramine 1 g/kg per day for 2 weeks by gavage. The serum bile acids level, serum cholesterol level and activity and mRNA expression of cholesterol 7-alpha-dydroxylase (CYP7A1) in liver tissues after treatment were assessed. The mRNA express... |
| |
| Keywords: | Cholic acids Cholestyramine Farnesoid X receptor Cholesterol 7-alpha-Hydroxylase |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
