| 骨骼肌肌纤维钙火花检测中的伪彩色增强 |
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| 引用本文： | 雷长海,杨勇骥,李东方,朱志年. 骨骼肌肌纤维钙火花检测中的伪彩色增强[J]. 医疗卫生装备, 2007, 28(7): 20-21 |
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| 作者姓名： | 雷长海 杨勇骥 李东方 朱志年 |
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| 作者单位： | 1. 第二军医大学基础部,上海市,200433
2. 第二军医大学生物物理研究所,上海市,200433 |
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| 摘 要： | 利用激光扫描共聚焦显微镜。对经Flou-3AM染色的、人工剥离的蟾蜍骨骼肌肌纤维进行连续扫描分析，捕获电刺激后骨骼肌肌纤维中发生的钙火花现象,并通过对检测结果进行伪彩色处理，将灰度图像转换为彩色图像，从而提高了检测结果的直观性,增强了对钙火花的表达能力。
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| 关 键 词： | 钙火花 共聚焦显微镜 伪色彩处理 |
| 文章编号： | 1003-8868（2007）07-0020-02 |
| 收稿时间： | 2007-04-13 |
| 修稿时间： | 2007-04-132007-06-15 |
Pseudo-color enhancement in calcium sparks detection of skeletal muscle fiber |
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| LEI Chang-hai,YANG Yong-ji,LI Dong-fang,ZHU Zhi-nian. Pseudo-color enhancement in calcium sparks detection of skeletal muscle fiber[J]. Chinese Medical Equipment Journal, 2007, 28(7): 20-21 |
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| Authors: | LEI Chang-hai YANG Yong-ji LI Dong-fang ZHU Zhi-nian |
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| Affiliation: | 1. Basic Department, the Second Military Medical University, Shanghai 200433, China; 2. Institute of Biophysics, the Second Military Medical University, Shanghai 200433, China |
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| Abstract: | Fluorescence probe Fluo-3AM can be luminary in confocal microscope after combining Ca2+,So nomadic Ca2+ in cytoplasm can be measured and tested by this way.Some confocal laser scan images are obtained after calcium in muscle fibers signed with F1uo-3AM. Then mapping function is used to convert the gray level image into colorful image by pseudo-color enhancement theory in digital image.Compared to the original gray level image,the processed pseudo-color image could be more easily distinguished.So the methods had a great value in Ca2+ detection fields. |
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| Keywords: | Ca2+ sparks confocal microscope pseudo-color enhancement |
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