人牙周膜成纤维细胞对米诺环素的跨膜转运

目的研究人牙周膜成纤维细胞（HPDLF）对米诺环素的跨膜转运，为通过根管局部及全身给药的假说提供实验依据。方法用米诺环素溶液孵育HPDLF和MC3T3- E1细胞，超声破碎细胞后，高效液相色谱法（HPLC）测定不同时间点的胞内药物含量，考马斯亮蓝法测定细胞蛋白总量。结果HPLC可以精确测量细胞内米诺环素的含量。细胞种类和孵育时间对细胞内米诺环素含量有显著性影响（P<0.01），胞内米诺环素含量随着时间延长而增加，两种细胞的胞内药物含量不同；胞外米诺环素浓度增加时，细胞内药物含量增加。结论米诺环素存在HPDLF的跨膜转运，这种转运与细胞外药物浓度及孵育时间有关，并存在着细胞差异。

Biological transport of minocycline by human periodontal ligament fibroblasts

Objective To investigate biological transport of minocycline by human periodontal ligament fibroblasts(HPDLF).To verify the hypothesis of delivering medicine to periodontium and the whole body through the root canal.Methods HPDLF and MC3T3-E1 cells were incubated in minocycline solutions.The intracellular antibiotics contents were measured by high performance liquid chromatography(HPLC) and the cell total protein was measured by Bradford protein assay.Results HPLC was an accurate,sensitive method for measurement of the intracellular minocycline.The incubation time and cell property had significant effect on the intracellular minocycline contens(P< 0.01).The intracellular contents increased with extracellular concentration.Conclusion Minocycline can be transported by HPDLF.The transport is concentration-dependent,time-dependent and cell specific.