A Simple Method for the Assay of Factor VIII |
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Authors: | BERGNA LUIS JOSE |
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Affiliation: | 1 Instituto de Investigaciones Hematológicas (Director Dr. A. Pavlovsky) de laAcademia Nacional de Medicina, Buenos Aires, Argentina.
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Abstract: | A simple method for the assay of factor VIII activity, based on the thromboplastin generation test of Biggs and Douglas, has been described.Bovine serum is used as a source of factor V and serum factors, and plasmafrom a hemophiliac is not required. The concentration of factor VIII of thetest plasma is compared to that of a standard normal human plasma. Theresults obtained with the method have been fairly reproducible. |
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