单核细胞增多性李斯特菌荧光定量PCR检测

目的　探索建立快速、敏感、特异的单核细胞增多性李斯特菌的PCR检验方法。方法　以单核细胞增多性李斯特菌hly A基因作为靶序列设计一对引物,并以单核细胞增多性李斯特菌标准菌株中提取的DNA作为模板,用常规PCR和荧光定量PCR对单核细胞增多性李斯特菌进行鉴定。结果　含有一段特异基因的6 0 0 bp片段,表现出良好的单核细胞增多性李斯特菌种特异性。结论　荧光定量PCR比普通PCR特异性强、灵敏度高、重现性好,可以定量、快捷地应用于检测单核细胞增多性李斯特菌。

An examination on Listeria monocytogenes with fluorescence quantitive PCR

Objective To set up a check-up approach of speediness,sensitiveness,specificity of Listeia monocytogenes by PCR examination.Methods A pair of primers were designed by hlyA gene as target sequence of Listeia monocytogenes,and the DNA from Listeia monocytogenes of standard bacterium strain was used as template and tested with general PCR and fluoresence quantitive PCR(FQ-PCR).Results A specific gene contained 600bp fragment confered good specific of Listeia monocytogenes.Conclusion FQ-PCR is better than general PCR in terms of distinctiveness,high sensitiveness and reliable repetition and thus can be applied into quantitative and swiftly checking up Listeia monocytogenes.