红芪总黄酮对过氧化氢致脐静脉内皮细胞损伤的抗氧化作用

目的:研究红芪总黄酮对过氧化氢(H2O2)诱导脐静脉内皮细胞损伤的保护作用。方法:化学发光法检测红芪总黄酮对氧自由基的清除能力。建立H2O2致脐静脉内皮细胞损伤模型,化学比色法检测乳酸脱氢酶(LDH),丙二醛(MDA)含量和超氧化物歧化酶(SOD)活性,单细胞凝胶电泳法检测对细胞DNA损伤的影响。结果:红芪总黄酮可清除氧自由基。100μmol/L H2O2诱导脐静脉内皮细胞4 h,细胞液LDH、细胞内MDA含量升高SOD活性降低,DNA损伤明显。红芪总黄酮可明显抑制MDA损伤,显著降低LDH释放及细胞内MDA含量,提高SOD活性。结论:红芪总黄酮对H2O2诱导脐静脉内皮细胞损伤具有保护作用,其作用机制可能与提高脐静脉内皮细胞的抗氧化能力有关。

Antioxidant Effect of Total Flavonoids of Hedysarum polybotry on Human Umbilical Vein Endothelial Cells Injury Induced by Hydrogen Peroxide

OBJECTIVE: To investigate protective effect of total flavonoids of Hedysarum polybotry on human umbilical vein endothelial cells injury induced by hydrogen peroxide. METHODS: Chemical light assay was used to detect the oxygen radicals scavenging capacity of total flavonoids of Hedysarum polybotry. Lactate dehydrogenase (LDH) in the culture medium, malondialdelyde (MDA) and superoxide dismutase (SOD) in the cell were measured by spectroscopy, respectively. The degress of DNA damage was evaluated with single cell gel electrophoresis (SCGE) technique. RESULTS: Total flavonoids of Hedysarum polybortry could scavenge oxygen radicals. After being exposed to 100 micromol/L H2O2 for 4 hours, the level of LDH and MDA was increased significantly while SOD level was decreased. However, total flavonoids of Hedysarum polybotry could decrease the content of LDH, MDA and increase SOD activity significantly and inhibit the damage of DNA. CONCLUSION: Total flavonoids of Hedysarum polybotry has protective effect on human umbilical vein endothelial cells injury induced by hydrogen peroxide,which may be related to increasing the antioxidant capacity of human umbilical vein endothelial cells.