低氧微环境对MDA-435细胞株MMP2、9及其抑制剂TIMP21表达的影响

目的：探讨低氧微环境对人乳腺癌细胞株MDA 435中基质金属蛋白酶(matrix metalloproteinases,MMPs)及其抑制剂(tissue inhibitors of matrix metalloproteinases，TIMPs)表达的影响。方法：分别置人乳腺癌细胞株MDA 435于常氧（21% O2、5% CO2）和低氧（1% O2、5% CO2和94% N2）环境中培养6?h，RT PCR技术mRNA水平检测MMP2、9及TIMP2、1的表达。结果：MDA 435细胞株表达MMP9和TIMP1 mRNA，未检测到MMP2和TIMP2 mRNA；低氧环境下MDA 435细胞株中MMP9 mRNA 水平显著上升(P＜0.05)，TIMP1基因的表达在低氧环境与常氧环境下差异无统计学意义(P＞0.05)。结论：与常氧环境相比，低氧环境下乳腺癌细胞株MDA 435中MMP9及其抑制剂TIMP1的表达平衡发生了改变，提示，进行肿瘤转移分子机制的体外研究应充分考虑氧环境的影响。

Effect of hypoxia on MMP2, 9 and TIMP2, 1 in human breast cancer cell line

Objective: To investigate the effect of hypoxia on the expressions of MMPs and TIMPs in human breast carcinoma cell line MDA-435.Methods: After the MDA-435 cells being cultured under normoxia(21% O_2,5% CO_2)or hypoxia(1% O_2,5% CO_2 and 94% N_2)for 6 hours,the expressions of MMP2,9 and TIMP2,1 were detected in mRNA level by RT-PCR.Results: The expressions of MMP2 and TIMP2 were not observed in human breast cancer cell line MDA-435;the expression of MMP9 increased significantly in mRNA levels when cells were cultured under normoxia(P<0.05),but no significant differences of TIMP1 were observed under normoxia or hypoxia(P>0.05).Conclusion: Hypoxia could affect the expression of MMP9 in human breast cancer cell line MDA-435,indicating that in vitro study on the molecule mechanism of neoplasm metastasis should take the oxygen environment into conclusion.