活性DNA诱导小鼠SLE样综合征的量效关系及其特点

目的：研究活性DNA的剂量与其诱导小鼠SLE样综合征的关系，并对其特点进行全面研究。方法：从ConA活化的小鼠脾淋巴细胞中提取DNA，以不同剂量的活性DNA免疫同系小鼠，用ELISA方法测定IgG类抗dsDNA、抗组蛋白抗体的动态变化以及产生抗体的亚型，用免疫荧光检测抗核抗体核型和肾脏免疫复合物沉积。结果：10μg活性DNA能100％诱导抗dsDNA、抗组蛋白抗体生成，诱导的抗体以IgGI类为主，且小鼠肾脏存在大量免疫复合物沉积。5μg活性DNA诱导小鼠ANA阳性率为25％。结论：10μg活性DNA即可诱导小鼠SLE样综合征，主要诱导自身体液免疫应答。

The dose-effect relationship between active DNA and SLE-Like syndrome and its characteristic

Objective:To study the relationship between the dose of active DNA and the induction of SLE-like syndrome.Methods: DNA from extracted ConA-activated spleen lymphocytes and immunized syngenic mice with different quantities of active DNA, the anti-dsDNA antibodies and anti-histone antibodies as well as the antibody subclass were detected by ELISA.The patterns of antinuclear antibodies and immune complexes in glomeruli were observed by immunofluorescent-stain. Results: 10 pig active DNA could induce all the animal to produce anti-dsDNA and anti-histone antibodies,and the induced autoann'bodies were mainly IgGl type.Only 25%animals produced autoantibodies immunized by 5 fjig active DNA. Conclusion:The minimum dose of active DNA to induce SLE-like syndrome was 10 ^tg,and it predominantly evoked the humoral response.