宁波市2004-2013年麻疹野病毒分离株的RT-PCR检测与分析

目的 分析宁波2004-2013年麻疹野病毒的分子生物学特征。方法 采用RT-PCR 对宁波2004-2013年分离的麻疹野毒株核蛋白基因碳末端450个核苷酸进行扩增，序列测定后与基因库中麻疹病毒各基因型参考株作比对分析。结果 宁波2004-2013年分离的31株麻疹病毒均为H基因型，其中6株(包括2004年1株及2005年5株)为H1b亚型，其它均为H1a亚型。基因同源性比对结果显示，31株麻疹病毒与H1a亚型参考株China93-4的同源性为97.1%～100%，与H1b亚型参考株China94-7的同源性为96.7%～100%，与中国疫苗株S191的核苷酸同源性为81.9％～92.4％，氨基酸同源性为87.2％～90.6％。结论 宁波2004-2013年麻疹病例主要由本土病毒株(H1亚型)传播引起，H1a基因亚型为绝对优势基因亚型，H1b亚型处于相对弱势，H1a亚型5个小分支病毒株引起的多个传播链造成麻疹野病毒在宁波广泛流行。

Wild-type measles viruses isolated in Ningbo,China, 2004-2013

In this study ,we analyzed the genetic characterizations and propagation paths of measles viruses prevalent in Ningbo ,China from 2004 to 2013 .Measles viruses were isolated from throat swab specimens from 2004 to 2013 ,and 450 bp fragments of C terminus of nucleoprotein （N） gene were amplified by RT-PCR .Sequence analysis was conducted of all 31 virus strains ,and then compared with other measles virus strains published in GenBank .Results showed that all the 31 measles virus isolates belonged to genotype H1 ,6 strains （one strains in 2004 and 5 ones in 2005） belonged to H1b subtype ,and others be-longed to H1a subtype .Compared the 31 strains to the China 93-4 and China 94-7 ,the homology of 450 bp fragments of C ter-minus of nucleoprotein （N） gene were 97 .1%-100% and 96 .7%-100% ,respectively .The homology of 450 bp fragments of nu-cleotide and nucleotide （amino acids） compared with S191 were 81 .9%-92 .4% and 87 .2%-90 .6% ,respectively .It’s suggested that genotype H1 measles virus circulated in Ningbo from 2004 to 2013 ,and H1a was the predominant epidemic strain ,and H1b strain was existed as well .There are five different transmission chains of H1a subtype caused measles co-circulations in Ning-bo .