雪莲花醇提物对大鼠组织和红细胞的抗氧化活性

 目的研究雪莲花醇提物(ESLHM)的抗氧化活性。方法用·HO生成系统Fe²⁺维生素C诱导大鼠心、肝、肾组织匀浆脂质过氧化,用TBA比色法测定MDA含量;用NBT还原法测酵母多糖A刺激大鼠中性粒细胞产生的O₂^-;用分光光度法测H₂O₂诱发的大鼠红细胞溶血度。结果对照组大鼠心、肝、肾匀浆经Fe²⁺+维生素C溶液诱发后MDA含量较空白组升高(P<0.01),中性粒细胞受酵母多糖A刺激后O₂^-生成较空白组升高(P<0.01),红细胞经H₂O₂诱发后溶血度较空白组升高(P<0.01)。而6.3,12.5,25,50,100,200μ·L^-1 ESLHM能不同程度抑制Fe²⁺维生素C诱导的大鼠心、肝、肾脂质过氧化产物MDA生成,其抑制心、肝、肾MDA生成的IC₅₀分别为29.4,32.9,31.4 μg·L^-1,其量效关系均呈负相关,相关系数分别为-0.905, -0.912,-0.908,相关性检验P值均<0.01;能不同程度抑制酵母多糖A刺激中性粒细胞生成O₂^-,其抑制中性粒细胞生成O₂-的IC₅₀为24.6 μg·L^-1,其量效关系呈负相关,相关系数为-0.887(P<0 01);能不同程度抑制H₂O₂诱发的红细胞氧化溶血,其抑制红细胞氧化溶血的IC₅₀为57.6μg·L^-1,其量效关系呈负相关,相关系数为-0.905(P<0.01),.结论雪莲花醇提物通过清除·HO,O₂^-及H₂O₂发挥抗氧化活性。

Antioxidation of ethanolic extract from Saussurea laniceps on tissues and erythrocytes in rats

OBJECTIVE To study antioxidation of ethanolic extract from Saussure laniceps Hand Mass (ESLHM) .METHODS The peroxidation of homogenates of rat heart,liver and kidney was induced by·HO generation system of Fe²⁺ and Vitamin C. Malondialdehyde (MDA)was determined using TBA colonmetric method. Superoxide(O₂^-)induced by zymosan-stimulated neutrophils was measured by NBT reduction method. H₂O₂-caused hemolysis of erythrocytes was measured spectrometrically.RESULTS Compared with controlgroup, MDA content significantly increased in the homogenates of heart, liver and kidney of rats induced by·HO generation system of Fe²⁺ and Vitamin C(P<0.01) . O₂^- generation significantly rose in zymosan-stimulated neutrophils of rats(P<0.01), hemolysis extent significantly increased in H₂O₂-caused erythrocytes of rats(P<0.01) . However, MDA formation in homogenates was inhibited by 6.3,12.5,25,50,100 and 200 μg·L^-1 ES-LHM and IC₅₀ in heart, liver and kidney respectively were 29.4,32.9 and 31.4 μg·L^-1, the relation between quantity and efficiency was negative and the relative coefficients were -0.905,-0.912 and -0.908 (all P<0.01) respectively. O₂^- generation from zymosan-stimulated neutrophils in rats was inhibited by ESLHM and IC₅₀ was 24.6 μg·L^-1,the relation between quantity and efficiency was negative and the relative coefficient was-0.887(P<0.01). H₂O₂-caused hemolysis of erythrocytes was inhibited by ESIUM and IC₅₀ was 57.6 μg·L^{-1, the relation between quantity and efficiency was negative and the relative coefficient was -0.905(P<0.01) .CONCLUSION ESLHM exhibited an antioxidation by scavenging ·HO and O₂-and H₂O₂ in biological system}