神经发育疗法对脑瘫患儿转化生长因子β1水平影响的研究

目的观察神经发育疗法（neurodevelopmental treatment，NDT）对脑瘫患儿血清转化生长因子β1（transforming growth factor-β1，TGF-β1）水平的影响。方法用双抗体夹心酶联免疫吸附试验法（ABCELISA法）分别检测脑瘫组NDT治疗前后和脑瘫高危新生儿（新生儿病例组）血清TGF-β1水平。结果新生儿病例组血清TGF-β1水平明显高于对照组、脑瘫组NDT治疗前后（P〈0．01）；脑瘫组NDT治疗前血清TGF-β1水平明显低于对照组和脑瘫组NDT治疗后（P〈0．01）；脑瘫组NDT治疗后血清TGF-β1水平明显高于对照组（P〈0．01）。结论TGF-β1在NDT治疗脑瘫过程中发挥重要的神经保护作用，并可以作为评估NDT疗效的生物学指标。

The effect of neurodevelopmental treatment on transforming growth factor-β1 levels in cerebral palsy patients

Objective To observe the effect of neurodevelopmental treatment （NDT） on transforming growth factor-β1 （TGF-V1） levels in cerebral palsy （CP） patients. Methods Serum samples of 27 CP patients, 21 neonates who suffered asphyxia and/or infection and 15 controls were obtained respectively and kept at - 40℃. Serum TGF-β1 levels were measured by the enzyme-linked immunosorbent assay double sandwich method （ABCELISA）. Results Serum TGF-β1 Levels in neonates were significantly higher than in controls,CP patients without NDT and CP patients with NDT（P〈0. 01 ） ;Serum TGF-β1 Levels in CP patients without NDT were significantly lower than in controls and CP patients with NDT（P〈0. 01）;Serum TGF-β1 Levels in CP patients with NDT were significantly higher than in controls（P〈0. 01 ）. Conclusions TGF-β1 plays an important role in CP patients with NDT. TGF-β1 can be used as an independent biomarker than reflects the curative effect of NDT.