蒙古族儿童过敏性紫癜与HLA—A基因的关联性

目的探索内蒙地区蒙古族儿童过敏性紫癜（AP）与HLA-A基因的关联性。可望找出相关基因，以探寻其部分发病机理及防治前景。方法采用病例对照研究策略，引入PCR—SSO技术，在祖籍三代居住内蒙地区，无血缘关系，无与异族通婚史及其他免疫性疾病史和家族史的蒙古族人群中，选择儿童AP病例组56例和健康儿童对照组66名，作HEA-A等位基因的型别分析。基因频率比较在单因素四格表X。或Fisher检验的基础上，作以Logistic多因素回归。结果病例组HLA-A$11基因频率为16．1％，与对照组的9．1％比较，Wald为3．954，P为0．047，差异有统计学意义。B为0．844〉0，OR为2．325〉1，促进发病，其95％可信区间为1．012—5．340，其内不包含1，与P意义相符，有统计学意义。EF为0．342〉0。结论HLA-A＊11等位基因可能是内蒙地区蒙古族儿童AP发病单体型中的一个遗传易感基因。

The correlation between HLA-A allele and anaphylactoid purpura in children of Mongolia

Objective To explore the association between HLA -A gene and anaphylactoid purpura(AP) in children of Mongolia in Inner Mongolia. To find correlated genes and study part of pathogenesis and the method of prevention and cure of AP. Methods The method of case control was adopted and selected 56 children with AP as case group and 66 health children as control group in Mongolia,who had resided in Inner Mongolia three generations without consanguinity, history of mixed, marriages, other medical history , and family history of immunity,led into polymerase chain reaction sequence specific oligonucleotide probes technique, analyzed the type of HLA-A gene. The compare of gene frenquency made with logistic regression after χ2 or Fisher test. Results The gene frenquency of HLA- A * 11 ( 16. 1% ) allele in case group compared to that of control group( 9. 1% ) ,Wald of HLA-A * 11 gene was 3. 954 ,P =0. 047, the difference had statistical significance. B = 0. 844 > 0, OR = 2. 325 > 1, it helped development of the disease,which 95％confident interval was 1. 012-5.340,which did not include 1 ,EF =0. 342 >0. Conclusion HLA-A * 11 allele may be the susceptible gene of AP in children of Mongolia in Inner Mongolia.