| 标本因素对荧光定量PCR法测定乙肝病毒DNA的影响 |
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| 引用本文： | 郑建中,王青,王凤华,王丽萍. 标本因素对荧光定量PCR法测定乙肝病毒DNA的影响[J]. 医学检验与临床, 2008, 19(5) |
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| 作者姓名： | 郑建中 王青 王凤华 王丽萍 |
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| 作者单位： | 山东邹城兖矿集团总医院输血科,邹城,273500;青岛市市立医院检验科,青岛,266011;山东邹城兖矿集团总医院检验科,邹城,273500 |
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| 摘 要： | 目的探讨脂血、溶血标本及血清标本常温储存48小时和4℃保存7天对乙肝病毒DNA(HBVDNA)荧光定量测定结果的影响。方法将乙肝大三阳血清标本进行即时检测、4℃保存7d后检测、室温保存48h检测及高脂血和非脂血、溶血血清和未溶血血清同时作HBVDNA荧光定量PCR,并两两间进行配对t检验。结果乙肝大三阳溶血与未溶血血清标本、脂血和非脂血血清标本HBVDNA含量都在同一数量级。血清标本在室温下短期贮存(48h内)、4℃保存7d内分别与即时检测HBV-DNA含量比较均无统计学意义(P>0.05)。结论脂血、溶血,血清标本不同储存温度及时间对HBV-DNA定量结果测定尤影响。
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| 关 键 词： | 荧光定量PCR 乙肝病毒DNA 标本 |
The influence of differente samples on fluoescebce qyabtutuve PCR of HBVDNA |
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| Zheng Jianzhong,Wang qing,Wang fenghua,Wang liping. The influence of differente samples on fluoescebce qyabtutuve PCR of HBVDNA[J]. Medical Laboratory Science and, 2008, 19(5) |
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| Authors: | Zheng Jianzhong Wang qing Wang fenghua Wang liping |
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| Affiliation: | Zheng Jianzhong,Wang liping (Department of Blood Transfution,General Hospital of YanKuang Co,Zoucheng,Sh,ong 173500) |
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| Abstract: | Objective To invesugate the effect of sample hemolysis,the high lipid, sera storage at 4℃ for 7 day storage at room temperature on fluorexence quantitive of HBV-DNA by real-time PCR detection. Method The serums from the case that were HBsAg+,HBeAg+ and anti-HBc+,the scums from the case first detections were performed as soon as the blood was drawn. The second and the third detections were done after the blood was stored at 4℃ for 7 days and at room temperature. The results of three detections were compared ... |
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| Keywords: | Fluorexcence quantitive PCR HBVDNA Sample |
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