口腔侵袭性鳞癌间质中CD34和α平滑肌肌动蛋白的表达

目的观察口腔侵袭性鳞癌间质中CD34和α平滑肌肌动蛋白（α-SMA）的表达。方法采用免疫组化SP法，检测60例口腔侵袭性鳞癌和其中20例切缘无瘤黏膜间质中CD34和α-SMA的表达。结果加例切缘无瘤黏膜间质中均有CD34的表达，主要位于口腔黏膜固有层、黏膜下层和血管旁的成纤维细胞胞质中，而无α-SMA的表达。α-SMA的表达仅见于血管壁平滑肌细胞。60例口腔侵袭性鳞癌间质中小血管壁内皮细胞中有CD34阳性表达，其中53例在肌成纤维细胞胞质中有α-SMA的表达。结论口腔侵袭性鳞癌间质中存在CD34表达缺失和α-SMA的表达获得现象，联合检测CD34和α-SMA有助于判断口腔癌的侵袭性。

Expression of stromal CD34 and α-smooth muscle actin in oral invasive cancer

OBJECTIVE: To observe the expression of stromal CD34 and alpha-smooth muscle actin (alpha-SMA) in oral invasive cancer. METHODS: The distribution and expression of stromal CD34 and alpha-SMA in 60 patients with invasive oral squamous cell cancer and 20 resections with tumor-free margins from 60 patients with invasive oral squamous cell cancer were examined by immunohistochemistry SP method. RESULTS: Twenty cases of resections of mucosa with tumor-free margins exhibited CD34 expression in fibrocytes cytoplasm in the vicinity of vessels, mucosa and submucosa but were free of alpha-SMA expression, only the walls of muscularized vessels stained positive for alpha-SMA in the stroma. Sixty invasive oral squamous cell carcinomas were free of stromal CD34 expression, only the endothelia of small vessels stained positive for CD34, of which 53 invasive oral squamous cell carcinomas expressed stromal alpha-SMA in myofibroblasts cytoplasm. CONCLUSIONS: The detection of CD34 and alpha-SMA is an adjunctive tool in judging oral cancer invasion in small oral mucosa biopsy specimens based on the absence of CD34 expression paralleled by the gain of alpha-SMA in the stroma of oral invasive cancer.