| CH50多肽在膀胱癌细胞系BIU-87中的表达和鉴定 |
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| 引用本文： | 伍庄,陈忠,叶章群,叶仕桥. CH50多肽在膀胱癌细胞系BIU-87中的表达和鉴定[J]. 医学研究生学报, 2005, 18(4): 306-310 |
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| 作者姓名： | 伍庄 陈忠 叶章群 叶仕桥 |
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| 作者单位： | 1. 华中科技大学同济医学院,同济医院泌尿外科,湖北,武汉,430030
2. 华中科技大学同济医学院,病理生理教研室,湖北,武汉,430030 |
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| 基金项目： | 武汉市科技攻关课题基金资助项目(批准号:20036004056) |
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| 摘 要： | 目的：研究CHS0多肽真核表达载体pCH510体外转染膀胱癌细胞系BIU-87以及CHSO多肽的表达和鉴定。方法：以脂质体Lipofectimine^TM2000为介导，将pCH510质粒体外转染给BIU-87细胞，用免疫组化S-P法、Western Blot法鉴定CH50多肽的表达，RT—PCR法鉴定导入基因的表达。结果：转染pCH510质粒的BIU-87细胞明显阳性表达CH50多肽，对照组则不表达。结论：BIU-87细胞体外转染pCH510质粒后能表达CH50多肽，改变了癌细胞的黏附属性，为临床治疗膀胱癌提供了新的途径。
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| 关 键 词： | CH50多肽 BIU-87细胞 转染 |
| 文章编号： | 1008-8199(2005)04-0306-03 |
| 修稿时间： | 2004-11-29 |
Expression and identify of CH50 polypeptide in bladder cancer cell line BIU-87 |
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| WU Zhuang,CHEN Zhong,YE Zhang-qun,YE Shi-qiao. Expression and identify of CH50 polypeptide in bladder cancer cell line BIU-87[J]. Bulletin of Medical Postgraduate, 2005, 18(4): 306-310 |
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| Authors: | WU Zhuang CHEN Zhong YE Zhang-qun YE Shi-qiao |
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| Affiliation: | WU Zhuang 1,CHEN Zhong 1,YE Zhang-qun 1,YE Shi-qiao 2 |
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| Abstract: | Objective: To investigate the expression and identification of CH50 polypeptide in bladder cancer cell line BIU-87 by gene transfection. Methods:The eukaryotic expressing vector pCH510 of polypeptide CH50 was introduced into BIU-87 cells by gene transfection Lipofectimine TM2000.The expressed product was identified by immunohistochemistry and Western blot method. The expression of the transfected gene was identified by RT-PCR. Results:The BIU-87 cells could produce CH50 polypeptide by transfection. Conclusion:When the vector of pCH510 was transfected into BIU-87 cells in vitro, the cell adhension characteristic would be changed. |
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| Keywords: | CH50 polypeptide BIU-87 cells Gene transfection |
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