脊髓缺血性损伤后神经元病变的神经元特异性烯醇酶研究

目的观察实验性兔腰髓缺血40分钟、再灌流4小时的脊髓运动神经元胞体和轴突的病理学改变。方法采用神经元特异性烯醇酶（NSE）抗体特异标记神经元胞体和轴突，其结果进行了图像分析。结果缺血40分钟，脊髓前角运动神经元胞体州轴突内NSE反应异常地增强，胞体内NSE分布紊乱聚集。再灌流4小时，脊髓前角运动神经完胞体和轴突内NSE反应阳性，胞体内NSE散乱、稀疏和溶解，轴突肿胀、扩大、消失。图像分析了脊髓前角运动神经元胞体内NSE的面积、发度和脊髓前索内轴突的数量，其结果具有显著性差异结论神经元特异性烯醇酶可作为神经元损伤的敏感标志物。

Neuron specific enolase immunohistochemical study of pathological changes of neurons in ischemic spinal cord injury

Objective To investigate the pathological changes of cell bodies and axons of motorneurons, Method Immunohistochemical method was used to observe the patholigical changes of cell bodies and axons of motorneurons in the lumbar spinal cord caused by 40 min ischemia and 4 h reperfusion in the rabbits. Neuron specific enolase (NSE) antibody was adopted to label cell bodies and axons specifically, and the results were analyzed by an image pattern analysis system. Result The excessive accumulation of NSE was observed in the cell bodies and axons of motorneurons in ventral horn after 40 min ischemia, and the distribution of NSE in the cell bodies was disturbance and accumulative The NSE immunohistochemical reaction could be shown in the cell bodies and axons of montorneurons after 4 h of reperfusion, and the distribution of NSE in the cell bodies was divergent, rare and disintegrative, the axons were swollen and vanish. The areas, grey levels of NSE in the cell bodies and the numbers of axons in the anterior funiculus were measured and the statistical analysis showed the significance difference. Conclusion NSE could be used as a sensitive marker for neuron injury.