体外循环中心肌细胞膜蛋白巯基结合位置的自旋标记——ESR研究

探讨缺血再灌注损伤对心肌细胞膜蛋白巯基的影响，评价四种心肌保护方法的保护作用。方法运用电子自旋共振技术（ＥＳＲ），研究体外循环中猫心肌细胞膜蛋白巯基结合位置的变化情况。结果在主动脉阻断期间和再灌注早期，各组Ｗ／Ｓ均显著减小，再灌注后期，各心肌保护组有不同程度恢复，其中未行心肌保护组减小最明显，常温体外循环温血停搏液持续顺灌组减小最小且恢复最佳。结论常温体外循环温血停搏液持续顺灌使心肌细胞膜蛋白流基得到最佳保护。

Spin label studies on the properties of sulfhydryl binding sites of myocardial membrane-proteins during cardiopulmonary bypass

Objective To study the ischemia/reperfusion-induced changes of sulfhydryl binding sites ofmyocardial membrane-proteins, and to evaluate the protective effects of four kinds of cardioplegia. MethodThe changes were studied by electric spin resonance (ESR). Result Significant decreases of W/S in all felineswere observed during aortic cross-clamping and early in reperfusion. After reperfusion, the W/S in the felinesreceiving cardioplegia began to return. The W/S was increased most significantly in the felines without administration of cardioplegia, while most slightly in the felines with continuous administration of warm blood cardioplegia. Conclusion The sulfhydryl of myocardial membrane-proteins is better protected by warm bloodcardioplegia than by others.