| PCR和探针杂交技术检测口腔粘膜疾病中人乳头瘤病毒的核酸 |
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| 引用本文： | 张捷 李呈瑞. PCR和探针杂交技术检测口腔粘膜疾病中人乳头瘤病毒的核酸[J]. 临床检验杂志, 1997, 15(3): 139-141 |
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| 作者姓名： | 张捷 李呈瑞 |
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| 作者单位： | 北京医科大学第三临床医院 |
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| 摘 要： | 联合应用ＰＣＲ和核酸杂交的方法对１５份口腔粘膜疾病患者样品进行了分析。用ＰＣＲ技术测ＨＰＶ－１６为３份阳性，ＨＰＶ－１８为７份阳性；然后用探针杂交检测ＰＣＲ产物其ＨＰＶ－１６，３份阳性，ＨＰＶ－１８，１０份阳性。以上结果表明ＨＰＶ与口腔粘膜疾病有一定的关系；用ＰＣＲ扩增法和探针杂交法联合检测可提高检测率。
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| 关 键 词： | 人类乳头瘤病毒 核酸杂交 聚合酶链反应 |
Examination of human popillomavirus of oral mucous membrane diseases by PCR and probe hybridization |
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| ZHANG Jie LI Changrui LIN Paifen. Examination of human popillomavirus of oral mucous membrane diseases by PCR and probe hybridization[J]. Chinese Journal of Clinical Laboratory Science, 1997, 15(3): 139-141 |
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| Authors: | ZHANG Jie LI Changrui LIN Paifen |
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| Abstract: | The correlation of Human Popillomavirus (HPV) with oral mucous membrane diseases was investigated. Method: HPV16, HPV18 was detected in 15 cases by using polymerase chain reaction(PCR) and dot blot hybridization. Results: 3 cases showed positive in HPV16 by both methods. 7 cases showed positive in HPV18 by PCR and 10 cases showed positive by dot blot hybridization. Conclusion: The results indicated correlation to HPV with oral mucous membrane disease. A high sensitivety and specifity was detected by using PCR and hybridization methods. |
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| Keywords: | Human papillomavirus(HPV) Nucleic acid hybridization Polymerase Chain Reaction(PCR) |
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