湿热环境下Ⅱ度烫伤大鼠施行冷却疗法后体核温度的改变

目的 观察在湿热环境下大鼠浅Ⅱ度烫伤创面即刻施行冷疗后体核温度的变化,同时评价冷疗敷料方法的降温效果。方法 24只Wistar大鼠随机分成4组,即常温常湿对照组、常温常湿冷疗组、湿热对照组和湿热冷疗组,每组6只。制作浅Ⅱ度烫伤模型后分别采取不同的干预条件,常温组的实验环境条件为干球温度(Tdb)(26.33±1.29)℃、相对湿度(rh)(71.05±4.57)%,高对组和高冷组的实验环境条件为Tdb(35.33±0.35)℃、rh(70.81±1.38)%,常对组和高对组不给予冷疗降温,常冷组和高冷组给予腹部冷疗敷料降温。暴露时间均为120 min。监测直肠温度(Tr),每20 min记录1次。结果 4组组内7个时间水平之间、时间水平组间及不同组间比较,湿热环境下大鼠Tr显著增高(P<0.001);冷疗组与对照组相比,Tr均有显著性降低(P<0.001),且环境温度和冷疗对Tr变化影响有交互作用(P=0.003)。随时间变化,Tr的变化趋势为常对组Tr缓慢下降、常冷组Tr有高低波动、湿热两组的Tr逐渐增加,Tr差值随时间逐渐增大。结论 湿热复合因素使烫伤大鼠的Tr逐渐增高;腹部冷疗敷料降温方法能有效降低大鼠的Tr,阻止热损伤的进程,实验证明腹部冷疗敷料降温是降低体温的较好方法

Rectal temperature changes of Wistar rats with second-degree scald burn in hot and humid environment following immediate cooling therapy

Objective To observe the changes in rectal temperature (Tr) after immediate cooling therapy for Wistar rats with superficial second-degree scald burn in hot and humid environment, and evaluate the effect of the dressing material for cooling. Methods Twenty-four Wistar rats were randomly divided into 4 groups (n=6), namely normal temperature control (NTC), normal temperature cooling therapy (NTCT), hot and humid control (HHC), and hot and humid cooling therapy (HHCT) groups. Superficial second-degree scald burns were induced in the rats, followed by interventions with cooling therapy in the two therapy groups at dry bulb temperature (Tdb) of 35.33±0.35 ℃ with relative humidity of 70.81%±1.38%, whereas the two control groups were treated at Tdb of 26.33±1.29 ℃ with relative humidity of 71.05%±4.57% without dressing for cooling therapy. The exposure time of each group was 120 min, and the Tr was recorded every 20 min. Results On the basis of comparisons between the measurements taken at 7 different time points, we found that the Tr of the rats was elevated in hot and humid environment (P<0.001) and decreased when cooling therapy was applied (P<0.001). Interactions between the environmental temperature and cooling therapy were noted in their influence on Tr (P=0.003). As the exposure time was prolonged, Tr slowly decreased in NTC group, mildly fluctuated in NTCT group, but elevated in HHC and HHCT groups with gradual increase of the differences between the measurements taken at the same time point. Conclusion Application of the dressing material on the abdomen for cooling therapy can efficiently lower the Tr, which may prevent the progression of the heat injury.