| GC-ECD法检测血浆中地西泮及去甲西泮 |
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| 引用本文： | 刘冬娴,贺江南.GC-ECD法检测血浆中地西泮及去甲西泮[J].西北药学杂志,2005,20(2):51-52. |
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| 作者姓名： | 刘冬娴 贺江南 |
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| 作者单位： | 湖南公安高等专科学校,湖南,长沙,410006 |
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| 摘 要： | 目的用GC-ECD法分析血浆中地西泮及其主要代谢物去甲西泮。方法血浆中加入内标去烃基氟西泮,调pH至10.8,用二氯甲烷-正己烷(7∶3)提取地西泮及去甲西泮,用GC-ECD法进行分析。结果检材中分析物的回收率80%以上,检出限5μg·L-1以下。结论该方法灵敏度高,可用于口服地西泮10mg人体24h内血浆的分析。
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| 关 键 词： | 地西泮 去甲西泮 气相色谱法 血浆 |
| 文章编号： | 1004-2407(2005)02-0051-02 |
| 修稿时间： | 2004年10月20 |
Determination of diazepam and nordiazepam in plasma by GC-ECD |
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| LIU Dong-xian,HE Jiang-nan.Determination of diazepam and nordiazepam in plasma by GC-ECD[J].Northwest Pharmaceutical Journal,2005,20(2):51-52. |
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| Authors: | LIU Dong-xian HE Jiang-nan |
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| Abstract: | Objective A method was developed for the determination of diazepam and its main metabolite, nordiazepam, in plasma by GC-ECD. Methods After adding norflurazepam(interior standard), diazepam and nordiazepam in plasma samples were extracted with dichloromethane-hexane (7∶3) at pH 10.8, and then determined by GC-ECD. Results The recoveries of the analytes in specimens were higher than 80% and the detection limits were below 5 μg·L -1. Conclusion This method is sensitive enough for the analysis of plasma from the subjects over a 24 hour period after receiving a 10mg dose of diazepam orally. |
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| Keywords: | Diazepam Nordiazepam Gas chromatography Plasma |
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