阻塞性睡眠呼吸暂停低通气综合征模式间歇低氧对大鼠淋巴细胞亚群凋亡的研究

目的 探讨睡眠呼吸暂停模式间歇低氧（IH）对大鼠外周血淋巴细胞亚群凋亡的影响.方法 将16只雄性Wistar大鼠随机分为常氧对照组（NC,n=8）和间歇低氧（IH）4周组（IH4,n=8）,IH暴露环境最低氧浓度为5％,IH频率为30次/h.暴露完成后取外周血分离淋巴细胞,流式细胞术检测淋巴细胞亚群凋亡情况.结果 和NC组比较,IH组CD4 T细胞凋亡率（15.17±1.43）％低于NC组（17.97±1.76）％,CD8 T细胞凋亡率（17.23±1.07）％低于NC组（19.7l±1.93）％,NK细胞凋亡率（22.07±1.45）％高于NC组（17.33±1.04）％,B细胞凋亡率（17.88±1.85）％高于NC组（13.94±1.76）％（P均＜0.01）.结论 IH暴露改变大鼠外周血淋巴细胞亚群的凋亡状态,导致免疫功能异常,可能加重内皮细胞功能障碍.

Effect of intermittent hypoxia on lymphocyte subset apoptosis in a mouse model of obstructive sleep apnea hypopnea syndrome

Objective To examine the effect of intermittent hypoxia （IH） on lymphocyte subset apoptosis in a mouse model of sleep apnea.Methods Sixteen male Wistar rats were randomly divided into 2groups （n =8 each）：normal oxygen control group （NC） and intermittent hypoxia for successive 4 weeks group （IH4）.For IH expose,the minimum oxygen concentrations was 5％ with an apnea hypopnea index of 30/h.Apoptosis of lymphocyte subset was detected using flow cytometry assay.Results Compared with the NC group,apoptosis of CD4 and CD8 T cells was decreased significantly in the IH4 group （P ＜ 0.01）,while apoptosis of B and NK cells increased significantly in the IH4 group （P ＜ 0.01）.Conclusion Intermittent hypoxia regulates differentially apoptosis in circulating blood lymphocyte subsets,which may lead to altered immunologic function and contribute to endothelial dysfunction.