稀释药物时抽吸无菌空气的研究

在同一治疗室内，分别采集密封无菌空瓶中、酒精灯蓝色火焰中和治疗室内空气，将以上三种空气分别注入无菌的１０％葡萄糖注射液中，在其滴注的不同时间作细菌培养；又将抽吸的这三种空气分别注入无菌的１０％葡萄糖注射液中，再各抽出无菌溶液稀释药物，将稀释药物与１０％葡萄糖注射液配伍后，在其滴注的不同时间作细菌培养。结果表明，密封无菌空瓶中、酒精灯蓝色火焰中抽吸的空气所稀释药物无细菌生氏；而治疗室内抽吸的空气稀释药物有细菌生长。提示：稀释药物抽吸空气应在密封无菌空瓶中采集；并在稀释药物过程中将此空气保留在无菌注射器针筒内再使用。

Study on drawing aseptic gas in diluting drugs

In this study,the gas was drawn from sealed aseptic bottles,the blue flame of an alcohol lamp,and the air of the same treatment room. And the gas was put into aseptic solutions of 10% glucose separately and dripped. Then the samples were taken for bacteriaculture at appointed time-points. Meanwhile, the gases were drawn and put into aseptic solutions of 10% glucose separtely. Then deactived penicillines were diluted with the solutions separately. Finally, the penicillines were mixed with 10% glucoses and dripped. The samples were taken for bacteria-culture in the same way. The results showed that there was no colony existed in the gas from the sealed aseptic bottles and the flame of the alcohol lamp. However,colonies existed in the samples from the air of the treatment room. It is suggested that sealed aseptic gas should be drawn and kept for use in diluting drugs.